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    Identification of a fall armyworm (Spodoptera frugiperda)-specific gene and development of a rapid and sensitive loop-mediated isothermal amplification assay

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    Journal Article (1.445Mb)
    Date
    2022
    Author
    Osabutey, A.F.
    Seo, B.Y.
    Kim, A.Y.
    Ha, T.A.T.
    Jung, J.
    Goergen, G.
    Owusu, E.O.
    Lee, G.S.
    Koh, Y.H.
    Type
    Journal Article
    Review Status
    Peer Review
    Target Audience
    Scientists
    Metadata
    Show full item record
    Abstract/Description
    The fall armyworm [FAW, Spodoptera frugiperda (J E Smith)], a moth native to America, has spread throughout the world since it was first discovered in Africa in 2016. The FAW is a polyphagous migratory pest that can travel over long distances using seasonal winds or typhoons because of its excellent flying ability, causing serious damage to many crops. For effective FAW control, accurate species identification is essential at the beginning of the invasion. In this study, the FAW-specific gene Sf00067 was discovered by performing bioinformatics to develop a fast and accurate tool for the species-specific diagnosis of this pest. An Sf00067 loop-mediated isothermal amplification (LAMP) assay was developed, and optimal conditions were established. The Sf00067 6 primer LAMP (Sf6p-LAMP) assay established in this study was able to diagnose various genotype-based strains of FAW captured in Korea and FAWs collected from Benin, Africa. Our FAW diagnostic protocol can be completed within 30 min, from the process of extracting genomic DNA from an egg or a 1st instar larva to species determination.
    https://dx.doi.org/10.1038/s41598-022-04871-2
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/7363
    IITA Authors ORCID
    Georg Goergenhttps://orcid.org/0000-0003-4496-0495
    Digital Object Identifier (DOI)
    https://dx.doi.org/10.1038/s41598-022-04871-2
    Research Themes
    Plant Production and Health
    IITA Subjects
    Agronomy; Food Security; Maize; Pests of Plants; Plant Breeding; Plant Production
    Agrovoc Terms
    Spodoptera Frugiperda; Genes; Dna; Pests of Plants; Maize; Pest Control
    Regions
    Africa; Asia; Southeast Asia; West Africa
    Countries
    Benin (Dahomey); Korea
    Hubs
    Headquarters and Western Africa Hub
    Journals
    Scientific Reports
    Collections
    • Journal and Journal Articles4835
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