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Datasets from harmonised metabolic phenotyping of root, tuber and banana crop
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Date
2022-06Type
Review Status
Peer ReviewTarget Audience
Scientists
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Biochemical characterisation of germplasm collections and crop wild relatives (CWRs) facilitates the assessment of biological potential and the selection of breeding lines for crop improvement. Data from the biochemical characterisation of staple root, tuber and banana (RTB) crops, i.e. banana (Musa spp.), cassava (Manihot esculenta), potato (Solanum tuberosum), sweet potato (Ipomoea batatas) and yam (Dioscorea spp.), using a metabolomics approach is presented. The data support the previously published research article “Metabolite database for root, tuber, and banana crops to facilitate modern breeding in understudied crops” (Price et al., 2020) [1]. Diversity panels for each crop, which included a variety of species, accessions, landraces and CWRs, were characterised. The biochemical profile for potato was based on five elite lines under abiotic stress. Metabolites were extracted from the tissue of foliage and storage organs (tuber, root and banana pulp) via solvent partition. Extracts were analysed via a combination of liquid chromatography – mass spectrometry (LC-MS), gas chromatography (GC)-MS, high pressure liquid chromatography with photodiode array detector (HPLC-PDA) and ultra performance liquid chromatography (UPLC)-PDA. Metabolites were identified by mass spectral matching to in-house libraries comprised from authentic standards and comparison to databases or previously published literature.
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Permanent link to this item
https://hdl.handle.net/20.500.12478/7410IITA Authors ORCID
Delphine Amahhttps://orcid.org/0000-0002-5706-8773
Ranjana Bhattacharjeehttps://orcid.org/0000-0002-5184-5930
Bettina Heiderhttps://orcid.org/0000-0002-9792-8512
Mathieu Rouardhttps://orcid.org/0000-0003-0284-1885
Rony Swennenhttps://orcid.org/0000-0002-5258-9043
Digital Object Identifier (DOI)
https://dx.doi.org/10.1016/j.dib.2022.108041