• Contact Us
    • Send Feedback
    • Login
    View Item 
    •   Home
    • Journal and Journal Articles
    • Journal and Journal Articles
    • View Item
    •   Home
    • Journal and Journal Articles
    • Journal and Journal Articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    Whole Repository
    CollectionsIssue DateRegionCountryHubAffiliationAuthorsTitlesSubject
    This Sub-collection
    Issue DateRegionCountryHubAffiliationAuthorsTitlesSubject

    My Account

    Login

    Welcome to the International Institute of Tropical Agriculture Research Repository

    What would you like to view today?

    Micropropagation and effect of phloroglucinol on rooting of Diospyros crassiflora Hiern

    Thumbnail
    View/Open
    Journal Article (649.9Kb)
    Date
    2020-04
    Author
    Tchouga, A.O.
    Deblauwe, V.
    Djabou, S.A.M.
    Forgione, G.
    Hanna, R.
    Niemenak, N.
    Type
    Journal Article
    Review Status
    Peer Review
    Target Audience
    Scientists
    Metadata
    Show full item record
    Abstract/Description
    The jet-black to streaked ebony wood produced by the African ebony (Diospyros crassiflora Hiern, Ebenaceae) is exploited in Central and West Africa. A conservation effort is currently underway in Cameroon to propagate the tree through seedlings and cuttings. However, the intermittent availability of its seeds and the long time required for rooting formation of cuttings are limiting its propagation. This study aims to develop a successful protocol for ebony micropropagation. In vitro culture of nodal segments from seedlings was performed in half-strength Murashige and Skoog (MS) medium supplemented with either zeatin (0.0, 2.3, 4.6, 9.1, 13.7, 18.2, 22.8, and 27.4 µm) or 6-benzylaminopurine (BAP) (0.0, 2.2, 4.4, 8.8, 13.3, 17.8, 22.2, and 26.6 µm). After 12 weeks, all media allowed shoot budbreak. Shoots displaying the greatest budbreak were observed with 22.8 µm zeatin and 22.2 µm BAP. The shoots were then transferred to a medium supplemented with indole-3-butyric acid (IBA) and phloroglucinol (PG) at different concentrations for root induction. Root induction was observed on the shoots initially induced in the medium with BAP, but not in those grown in the medium with zeatin. In half-strength MS supplemented with 396.5 µm PG plus 14.2 µm IBA, the formation of a single tap root was observed on 79% of shoots, with an average root length of 2.8 ± 0.18 cm. Rooted plants were successfully acclimatized to the greenhouse, with a 50% survival rate. This is the first report on Diospyros crassiflora micropropagation, which paves the way for rapid ebony multiplication to respond to needed conservation efforts.
    https://dx.doi.org/10.21273/hortsci14556-19
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/7434
    IITA Authors ORCID
    Vincent Deblauwehttps://orcid.org/0000-0001-9881-1052
    Rachid Hannahttps://orcid.org/0000-0002-5715-0144
    Digital Object Identifier (DOI)
    https://dx.doi.org/10.21273/hortsci14556-19
    Research Themes
    Natural Resource Management
    IITA Subjects
    Agronomy; Forestry; Natural Resource Management; Plant Production
    Agrovoc Terms
    In Vitro Culture; Microcuttings; Plant Growth Substances; Micropropagation
    Regions
    Africa; Central Africa
    Countries
    Cameroon
    Hubs
    Central Africa Hub
    Journals
    HortScience
    Collections
    • Journal and Journal Articles4835
    copyright © 2019  IITASpace. All rights reserved.
    IITA | Open Access Repository