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    Differential root transcriptomics in a polyploid non-model crop: the importance of respiration during osmotic stress

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    U16ArtZorrillafontanesiDifferentialInthomNodev.pdf (1.142Mb)
    Date
    2016-03-03
    Author
    Zorrilla-Fontanesi, Y.
    Rouard, M.
    Cenci, A.
    Kissel, E.
    Do, H.
    Dubois, E.
    Nidelet, S.
    Roux, N.
    Swennen, R.L.
    Carpentier, S.C.
    Type
    Journal Article
    Target Audience
    Scientists
    Metadata
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    Abstract/Description
    To explore the transcriptomic global response to osmotic stress in roots, 18 mRNA-seq libraries were generated from three triploid banana genotypes grown under mild osmotic stress (5% PEG) and control conditions. Illumina sequencing produced 568 million high quality reads, of which 70–84% were mapped to the banana diploid reference genome. Using different uni- and multivariate statistics, 92 genes were commonly identified as differentially expressed in the three genotypes. Using our in house workflow to analyze GO enriched and underlying biochemical pathways, we present the general processes affected by mild osmotic stress in the root and focus subsequently on the most significantly overrepresented classes associated with: respiration, glycolysis and fermentation. We hypothesize that in fast growing and oxygen demanding tissues, mild osmotic stress leads to a lower energy level, which induces a metabolic shift towards (i) a higher oxidative respiration, (ii) alternative respiration and (iii) fermentation. To confirm the mRNA-seq results, a subset of twenty up-regulated transcripts were further analysed by RT-qPCR in an independent experiment at three different time points. The identification and annotation of this set of genes provides a valuable resource to understand the importance of energy sensing during mild osmotic stress.
    https://dx.doi.org/10.1038/srep22583
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/765
    Digital Object Identifier (DOI)
    https://dx.doi.org/10.1038/srep22583
    IITA Subjects
    Banana
    Agrovoc Terms
    Osmotic Stress; Bananas; Drought Stress; Drought Tolerance
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    Scientific Reports
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