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    Genome sequence of the banana aphid, Pentalonia nigronervosa Coquerel (Hemiptera: Aphididae) and its symbionts

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    Journal Article (1.060Mb)
    Date
    2020-12-01
    Author
    Mathers, T.C.
    Mugford, S.T.
    Hogenhout, S.A.
    Tripathi, L.
    Type
    Journal Article
    Review Status
    Peer Review
    Target Audience
    Scientists
    Metadata
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    Abstract/Description
    The banana aphid, Pentalonia nigronervosa Coquerel (Hemiptera: Aphididae), is a major pest of cultivated bananas (Musa spp., order Zingiberales), primarily due to its role as a vector of Banana bunchy top virus (BBTV), the most severe viral disease of banana worldwide. Here, we generated a highly complete genome assembly of P. nigronervosa using a single PCR-free Illumina sequencing library. Using the same sequence data, we also generated complete genome assemblies of the P. nigronervosa symbiotic bacteria Buchnera aphidicola and Wolbachia. To improve our initial assembly of P. nigronervosa we developed a k-mer based deduplication pipeline to remove genomic scaffolds derived from the assembly of haplotigs (allelic variants assembled as separate scaffolds). To demonstrate the usefulness of this pipeline, we applied it to the recently generated assembly of the aphid Myzus cerasi, reducing the duplication of conserved BUSCO genes by 25%. Phylogenomic analysis of P. nigronervosa, our improved M. cerasi assembly, and seven previously published aphid genomes, spanning three aphid tribes and two subfamilies, reveals that P. nigronervosa falls within the tribe Macrosiphini, but is an outgroup to other Macrosiphini sequenced so far. As such, the genomic resources reported here will be useful for understanding both the evolution of Macrosphini and for the study of P. nigronervosa. Furthermore, our approach using low cost, high-quality, Illumina short-reads to generate complete genome assemblies of understudied aphid species will help to fill in genomic black spots in the diverse aphid tree of life.
    https://dx.doi.org/10.1534/g3.120.401358
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/7728
    IITA Authors ORCID
    Leena Tripathihttps://orcid.org/0000-0001-5723-4981
    Digital Object Identifier (DOI)
    https://dx.doi.org/10.1534/g3.120.401358
    Research Themes
    Biotech and Plant Breeding
    IITA Subjects
    Agronomy; Banana; Pests of Plants; Plant Breeding; Plant Diseases; Plant Production
    Agrovoc Terms
    Hemiptera; Genomes; Vectors; insects; Pests of Plants; Bananas
    Regions
    Africa; East Africa
    Countries
    Kenya
    Hubs
    Eastern Africa Hub
    Journals
    G3-Genes Genomes Genetics
    Collections
    • Journal and Journal Articles4835
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