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    Optimized protocol for in vitro pollen germination in yam (Dioscorea spp.)

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    Journal Article (2.158Mb)
    Date
    2021
    Author
    Mondo, J.M.
    Agre, P.A.
    Asiedu, R.
    Akoroda, M.O.
    Asfaw, A.
    Type
    Journal Article
    Review Status
    Peer Review
    Target Audience
    Scientists
    Metadata
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    Abstract/Description
    Yam (Dioscorea spp.) plants are mostly dioecious and sometimes monoecious. Low, irregular, and asynchronous flowering of the genotypes are critical problems in yam breeding. Selecting suitable pollen parents and preserving yam pollen for future use are potential means of controlling these constraints and optimizing hybridization practice in yam breeding programs. However, implementing such procedures requires a robust protocol for pollen collection and viability testing to monitor pollen quality in the field and in storage. This study, therefore, aimed at optimizing the pollen germination assessment protocol for yam. The standard medium composition was stepwisely modified, the optimal growth condition was tested, and in vivo predictions were made. This study showed that the differences in yam pollen germination percentage are primarily linked to the genotype and growing conditions (i.e., medium viscosity, incubation temperature, and time to use) rather than the medium composition. The inclusion of polyethylene glycol (PEG) in the culture medium caused 67–75% inhibition of germination in D. alata. Although the in vivo fertilization was dependent on female parents, the in vitro germination test predicted the percentage fruit set at 25.2–79.7% and 26.4–59.7% accuracy for D. rotundata and D. alata genotypes, respectively. This study provides a reliable in vitro yam pollen germination protocol to support pollen management and preservation efforts in yam breeding.
    https://dx.doi.org/10.3390/plants10040795
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/7771
    IITA Authors ORCID
    Paterne AGREhttps://orcid.org/0000-0003-1231-2530
    Robert Asieduhttps://orcid.org/0000-0001-8943-2376
    Asrat Asfawhttps://orcid.org/0000-0002-4859-0631
    Digital Object Identifier (DOI)
    https://dx.doi.org/10.3390/plants10040795
    Research Themes
    Biotech and Plant Breeding
    IITA Subjects
    Agronomy; Plant Breeding; Plant Production; Yam
    Agrovoc Terms
    Dioscorea Rotundata; Dioscorea Alata; Genotypes; Varieties; Storage; Fertilization
    Regions
    Africa; West Africa
    Countries
    Nigeria
    Hubs
    Headquarters and Western Africa Hub
    Journals
    Plants
    Collections
    • Journal and Journal Articles4835
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