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A molecular survey of bacterial species in the guts of black soldier fly larvae (Hermetia illucens) reared on two urban organic waste streams in Kenya

dc.contributor.authorShumo, M.
dc.contributor.authorKhamis, F.M.
dc.contributor.authorOmbura, F.L.O.
dc.contributor.authorTanga, C.M.
dc.contributor.authorFiaboe, K.
dc.contributor.authorSubramanian, S.
dc.contributor.authorEkesi, S.
dc.contributor.authorSchluter, O.K.
dc.contributor.authorHuis, A.V.
dc.contributor.authorBorgemeister, C.
dc.date.accessioned2022-09-21T09:55:06Z
dc.date.available2022-09-21T09:55:06Z
dc.date.issued2021-09-22
dc.identifier.citationShumo, M., Khamis, F.M., Ombura, F.L.O., Tanga, C.M., Fiaboe, K., Subramanian, S., ... & Borgemeister, C. (2021). A molecular survey of bacterial species in the guts of black soldier fly larvae (Hermetia illucens) reared on two urban organic waste streams in Kenya. Frontiers in Microbiology, 12: 687103, 1-15.
dc.identifier.issn1664-302X
dc.identifier.urihttps://hdl.handle.net/20.500.12478/7788
dc.description.abstractGlobally, the expansion of livestock and fisheries production is severely constrained due to the increasing costs and ecological footprint of feed constituents. The utilization of black soldier fly (BSF) as an alternative protein ingredient to fishmeal and soybean in animal feed has been widely documented. The black soldier fly larvae (BSFL) used are known to voraciously feed and grow in contaminated organic wastes. Thus, several concerns about their safety for inclusion into animal feed remain largely unaddressed. This study evaluated both culture-dependent sequence-based and 16S rDNA amplification analysis to isolate and identify bacterial species associated with BSFL fed on chicken manure (CM) and kitchen waste (KW). The bacteria species from the CM and KW were also isolated and investigated. Results from the culture-dependent isolation strategies revealed that Providencia sp. was the most dominant bacterial species detected from the guts of BSFL reared on CM and KW. Morganella sp. and Brevibacterium sp. were detected in CM, while Staphylococcus sp. and Bordetella sp. were specific to KW. However, metagenomic studies showed that Providencia and Bordetella were the dominant genera observed in BSFL gut and processed waste substrates. Pseudomonas and Comamonas were recorded in the raw waste substrates. The diversity of bacterial genera recorded from the fresh rearing substrates was significantly higher compared to the diversity observed in the gut of the BSFL and BSF frass (leftovers of the rearing substrates). These findings demonstrate that the presence and abundance of microbiota in BSFL and their associated waste vary considerably. However, the presence of clinically pathogenic strains of bacteria in the gut of BSFL fed both substrates highlight the biosafety risk of potential vertical transmission that might occur, if appropriate pre-and-postharvest measures are not enforced.
dc.description.sponsorshipCanadian International Development Research Centre
dc.description.sponsorshipAustralian Centre for International Agricultural Research
dc.description.sponsorshipGerman Ministry of Economic Cooperation and Development
dc.description.sponsorshipNetherlands Organization for Scientific Research
dc.description.sponsorshipInternational Centre of Insect Physiology and Ecology
dc.description.sponsorshipUK Aid
dc.description.sponsorshipSwedish International Development Cooperation Agency
dc.description.sponsorshipSwiss Agency for Development and Cooperation
dc.description.sponsorshipFederal Ministry for Economic Cooperation and Development (BMZ), Germany
dc.description.sponsorshipKenyan Government
dc.format.extent1-15
dc.language.isoen
dc.subjectOrganic Wastes
dc.subjectWaste Treatment
dc.subjectHermetia Illucens
dc.subjectMicrobial Flora
dc.subjectInsect Rearing
dc.subjectFeed Safety
dc.subjectFood Security
dc.subjectKenya
dc.titleA molecular survey of bacterial species in the guts of black soldier fly larvae (Hermetia illucens) reared on two urban organic waste streams in Kenya
dc.typeJournal Article
cg.contributor.affiliationLeibniz-Institute for Agricultural Engineering Potsdam-Bornim
cg.contributor.affiliationCenter for Development Research (ZEF), Germany
cg.contributor.affiliationInternational Centre of Insect Physiology and Ecology
cg.contributor.affiliationInsect Technology Center (ITC), Germany
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationWageningen University and Research Centre
cg.coverage.regionAfrica
cg.coverage.regionEast Africa
cg.coverage.countryKenya
cg.coverage.hubCentral Africa Hub
cg.identifier.bibtexciteidSHUMO:2021
cg.isijournalISI Journal
cg.authorship.typesCGIAR and advanced research institute
cg.iitasubjectAgronomy
cg.iitasubjectBiodiversity
cg.iitasubjectFood Security
cg.iitasubjectPlant Breeding
cg.iitasubjectPlant Diseases
cg.iitasubjectPlant Health
cg.iitasubjectPlant Production
cg.journalFrontiers in Microbiology
cg.notesPublished online: 22 Sep 2021
cg.accessibilitystatusOpen Access
cg.reviewstatusPeer Review
cg.usagerightslicenseCreative Commons Attribution 4.0 (CC BY 0.0)
cg.targetaudienceScientists
cg.identifier.doihttps://dx.doi.org/10.3389/fmicb.2021.687103
cg.iitaauthor.identifierKomi Fiaboe: 0000-0001-5113-2159
cg.futureupdate.requiredNo
cg.identifier.volume12
cg.contributor.acknowledgementsThe authors are extremely thankful to their esteemed colleague Guido Luechters (ZEF) for his valuable assistance in the data analysis andMaureen Ong’onge, Joseph Gichuhi, Isaiah Rachami, and Dennis Muthoni (ICIPE) for their technical assistance. The authors are also grateful to the reviewers whose comments and suggestions helped shape this manuscript.


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