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    Potential applications of the CRISPR/Cas technology for genetic improvement of yam (Dioscorea spp.)

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    Journal Article (1.379Mb)
    Date
    2021-09
    Author
    Syombua, E.D.
    Tripathi, J.
    Obiero, G.
    Nguu, E.
    Yang, B.
    Wang, K.
    Tripathi, L.
    Type
    Journal Article
    Review Status
    Peer Review
    Target Audience
    Scientists
    Metadata
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    Abstract/Description
    Yam (Dioscorea spp.) is an economically important crop grown in the tropical and subtropical regions, producing tuberous roots that serve as a staple food, an income source, and an excellent source of various pharmaceutical precursors. Yam production is constrained by disease and pest infestations and a range of abiotic stresses. Genetic improvement can significantly mitigate these challenges, improve productivity, expand the yam markets, and increase economic gains. However, several intrinsic attributes of the crop have curtailed progress in yam breeding. Advanced genetic engineering such as genome editing by sequence-specific nucleases has emerged as complementary approaches to conventional breeding techniques. Mainly, the clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas) system for genome editing has provided robust platforms for gene function analysis and crop improvement in the post-genomic era. Despite its significance, research towards improving the yam species remains under-represented compared to other staple tuber crops such as cassava and sweet potato. Thus, it is critical to explore avenues for increasing the genetic gains from this under-exploited crop. The present review focuses on the progress and prospects for applying the CRISPR/Cas technology for yam improvement. The study elaborates on the currently available CRISPR/Cas tool for yam genome engineering and explores the potential applications of this toolkit in mitigating the various challenges encountered in yam production and consumption. Furthermore, we have delved into the challenges associated with this technology and the improvements made to minimize these challenges. The insights presented herein provide a guide for yam improvement to increase genetic gains from this under-researched and under-utilized resource.
    Acknowledgements
    The authors wish to thank the US National Science Foundation (IOS 1543888 to KW, LT, and BY).
    https://dx.doi.org/10.1002/fes3.330
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/7791
    IITA Authors ORCID
    Jaindra Tripathihttps://orcid.org/0000-0002-6366-917X
    Leena Tripathihttps://orcid.org/0000-0001-5723-4981
    Digital Object Identifier (DOI)
    https://dx.doi.org/10.1002/fes3.330
    Research Themes
    Biotech and Plant Breeding
    IITA Subjects
    Agronomy; Food Security; Genetic Improvement; Plant Breeding; Plant Health; Plant Production; Yam
    Agrovoc Terms
    Dioscorea; Genomes; Gene Editing; Yams; Improvement
    Regions
    Africa; East Africa
    Countries
    Kenya
    Hubs
    Eastern Africa Hub
    Journals
    Food and Energy Security
    Collections
    • Journal and Journal Articles4835
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