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First report of banana bunchy top virus in banana and plantain (Musa spp.) in Tanzania

dc.contributor.authorMpoki, S.
dc.contributor.authorMahuku, G.
dc.contributor.authorMbanzibwa, D.R.
dc.contributor.authorMkamilo, G.
dc.contributor.authorMark, D.
dc.contributor.authorMosha, H.I.
dc.contributor.authorPallangyo, B.
dc.contributor.authorFihavango, M.
dc.contributor.authorOresanya, A.
dc.contributor.authorOgunsanya, P.
dc.contributor.authorKumar, P.L.
dc.date.accessioned2022-09-22T07:49:03Z
dc.date.available2022-09-22T07:49:03Z
dc.date.issued2022-04
dc.identifier.citationMpoki, S., Mahuku, G., Mbanzibwa, D.R., Mkamilo, G., Mark, D., Mosha, H., ... & Kumar, P.L. (2021). First report of banana bunchy top virus in banana and plantain (Musa spp.) in Tanzania. Plant Disease, 106(4):1312, 1-7.
dc.identifier.issn0191-2917
dc.identifier.urihttps://hdl.handle.net/20.500.12478/7792
dc.description.abstractBanana (including plantain; Musa spp.) is an important vegetatively propagated food staple grown as a semi-perennial crop in fields and backyard gardens in Tanzania. Banana bunchy top disease (BBTD), caused by the banana bunchy top virus (BBTV, genus Babuvirus), is the most economically important viral disease of banana, infection of which results in severe stunting and reduction in fruit production by 90-100% within two seasons. The virus is spread by the banana aphid, Pentalonia nigronervosa, and through vegetative propagation of infected sources. BBTV is an introduced virus first reported in sub-Saharan Africa (SSA) in the 1960s in the Democratic Republic of Congo. Since then, BBTV spread was confirmed in 15 countries in Central, Southern, and Western African regions but was not detected in any previous surveys in the East African sub-region. During banana pests and disease surveys conducted in December 2020 – January 2021 in Buhigwe District in the Kigoma Region of Tanzania revealed banana plants with typical BBTV symptoms (severe stunting, leaves with shortened petioles, chlorotic streaks, and yellow leaf margins) in several banana fields in Muhinda (lon. 29.78662, lat. -4.53672) and Mwayaya (lon. 29.8218, lat. -4.49203) villages. Most of the affected plantations were 5 to 15 years old. Leaf samples (N=21) from symptomatic (N=6) and asymptomatic (N=15) banana plants were collected and used for total DNA extraction and BBTV detection by polymerase chain reaction (PCR) using the primer pair BBTV-1 and BBTV-2 to amplify ~240 bp sequence of DNA-R encoding for core master replication initiator protein gene. All samples from symptomatic plants tested positive and asymptomatic plants were negative. To further confirm the virus identity, four samples, each from symptomatic (PCR positive) and asymptomatic (PCR negative) plants from Muhinda and Mwayaya villages, were tested by Triple Antibody Sandwich-Enzyme-linked Immunosorbent Assay (TAS-ELISA) using BBTV ELISA reagent set (Cat. # SRA24700-1000, Agdia, France) following the manufacturer's protocol. Samples from symptomatic plants reacted positively in TAS-ELISA, and asymptomatic plants were negative. The 240-bp PCR product of two isolates was purified, and both strands were sequenced. A BLAST search of the nucleotide sequences (NCBI GenBank Acc.# MW711671 and MW711672) revealed 99% identity with DNA-R sequences of several other BBTV isolates from Africa (Acc. No# JF755994). Further analysis of the 240-bp nucleotide sequences with Maximum-likelihood phylogenetic analysis using MEGA-X software has grouped the two BBTV sequence isolates with the SSA sub-clade of the South-Pacific group. To our knowledge, this is the first report of BBTV infecting bananas in Tanzania, and East Africa endowed with rich banana diversity and popular East African Highland banana clone. BBTV presents a new threat to banana production in this sub-region due to the high risk of further spread through vegetative propagation, traditional planting material exchange practices, and the ubiquitous banana aphid vector. This study warrants delimitation surveys to assess the extent of spread, with simultaneous efforts to raise awareness about BBTD recognition and control measures among banana growers, including eradicating infected mats and replanting with healthy planting material to recover banana production.
dc.format.extent1-7
dc.language.isoen
dc.subjectBananas
dc.subjectPlantains
dc.subjectDiseases
dc.subjectBunchy Top Disease
dc.subjectDna
dc.subjectSubsaharan Africa
dc.subjectTanzania
dc.titleFirst report of banana bunchy top virus in banana and plantain (Musa spp.) in Tanzania
dc.typeJournal Article
cg.contributor.crpAgriculture for Nutrition and Health
cg.contributor.crpMaize
cg.contributor.crpRoots, Tubers and Bananas
cg.contributor.affiliationTanzania Agricultural Research Institute
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationMinistry of Agriculture, Livestock and Fisheries, Tanzania
cg.coverage.regionAfrica
cg.coverage.regionEast Africa
cg.coverage.countryTanzania
cg.coverage.hubEastern Africa Hub
cg.coverage.hubHeadquarters and Western Africa Hub
cg.researchthemePlant Production and Health
cg.identifier.bibtexciteidMPOKI:2021
cg.isijournalISI Journal
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectAgronomy
cg.iitasubjectBanana
cg.iitasubjectDisease Control
cg.iitasubjectFood Security
cg.iitasubjectPlant Breeding
cg.iitasubjectPlant Diseases
cg.iitasubjectPlant Health
cg.iitasubjectPlant Production
cg.iitasubjectPlantain
cg.journalPlant Disease
cg.notesPublished online: 27 Sep 2021
cg.accessibilitystatusOpen Access
cg.reviewstatusPeer Review
cg.usagerightslicenseCreative Commons Attribution 4.0 (CC BY 0.0)
cg.targetaudienceScientists
cg.identifier.doihttps://dx.doi.org/10.1094/pdis-07-21-1387-pdn
cg.iitaauthor.identifierGeorge Mahuku: 0000-0001-8444-8651
cg.iitaauthor.identifierP. Lava Kumar: 0000-0003-4388-6510
cg.futureupdate.requiredNo
cg.identifier.issue4
cg.identifier.volume106


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