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dc.contributor.authorNavarro, B.
dc.contributor.authorLi, S.
dc.contributor.authorGisel, A.
dc.contributor.authorChiumenti, M.
dc.contributor.authorMinutolo, M.
dc.contributor.authorAlioto, D.
dc.contributor.authorDi Serio, F.
dc.date.accessioned2022-12-01T10:58:04Z
dc.date.available2022-12-01T10:58:04Z
dc.date.issued2022
dc.identifier.citationNavarro, B., Li, S., Gisel, A., Chiumenti, M., Minutolo, M., Alioto, D. & Di Serio, F. (2022). A novel self-cleaving viroid-like RNA identified in RNA preparations from a citrus tree is not directly associated with the plant. Viruses, 14(10): 2265, 1-14.
dc.identifier.issn1999-4915
dc.identifier.urihttps://hdl.handle.net/20.500.12478/7949
dc.description.abstractViroid and viroid-like satellite RNAs are infectious, circular, non-protein coding RNAs reported in plants only so far. Some viroids (family Avsunviroidae) and viroid-like satellite RNAs share self-cleaving activity mediated by hammerhead ribozymes (HHRzs) endowed in both RNA polarity strands. Using a homology-independent method based on the search for conserved structural motifs of HHRzs in reads and contigs from high-throughput sequenced RNAseq libraries, we identified a novel small (550 nt) viroid-like RNA in a library from a Citrus reticulata tree. Such a viroid-like RNA contains a HHRz in both polarity strands. Northern blot hybridization assays showed that circular forms of both polarity strands of this RNA (tentatively named citrus transiently-associated hammerhead viroid-like RNA1 (CtaHVd-LR1)) exist, supporting its replication through a symmetric pathway of the rolling circle mechanism. CtaHVd-LR1 adopts a rod-like conformation and has the typical features of quasispecies. Its HHRzs were shown to be active during transcription and in the absence of any protein. CtaHVd-LR1 was not graft-transmissible, and after its first identification, it was not found again in the original citrus source when repeatedly searched in the following years, suggesting that it was actually not directly associated with the plant. Therefore, the possibility that this novel self-cleaving viroid-like RNA is actually associated with another organism (e.g., a fungus), in turn, transiently associated with citrus plants, is proposed.
dc.description.sponsorshipEuropean Union
dc.format.extent1-14
dc.language.isoen
dc.subjectRna
dc.subjectViroids
dc.subjectCitrus Reticulata
dc.titleA novel self-cleaving viroid-like RNA identified in RNA preparations from a citrus tree is not directly associated with the plant
dc.typeJournal Article
cg.contributor.affiliationInstitute for Sustainable Plant Protection
cg.contributor.affiliationJiangsu Academy of Agricultural Sciences
cg.contributor.affiliationIstituto di Tecnologie Biomediche. Bari, Italy
cg.contributor.affiliationInternational Institute of Tropical Agriculture,
cg.contributor.affiliationUniversità degli Studi di Napoli
cg.coverage.hubHeadquarters and Western Africa Hub
cg.researchthemeBiotech and Plant Breeding
cg.identifier.bibtexciteidNAVARRO:2022
cg.isijournalISI Journal
cg.authorship.typesCGIAR and advanced research institute
cg.iitasubjectBiodiversity
cg.iitasubjectBioscience
cg.journalViruses
cg.notesOpen Access Article; Published online: 15 Oct 2022
cg.accessibilitystatusOpen Access
cg.reviewstatusPeer Review
cg.usagerightslicenseCreative Commons Attribution 4.0 (CC BY 0.0)
cg.targetaudienceScientists
cg.identifier.doihttps://dx.doi.org/10.3390/v14102265
cg.iitaauthor.identifierANDREAS GISEL: 0000-0001-7218-9488
cg.futureupdate.requiredNo
cg.identifier.issue10
cg.identifier.volume14
cg.contributor.acknowledgementsThis work is dedicated to the memory of Giovanni Paolo Martelli, an example of a true visionary scientist, and for some of us, irreplaceable mentor and guide. We had the privilege of meeting him almost daily and sharing ideas and projects with him. We also had the opportunity to appreciate his human qualities and his innate ability to see beyond, guiding everyone in the right direction to accommodate their scientific curiosities.


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