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dc.contributor.authorWachira, J.M.
dc.contributor.authorKiplimo, D.
dc.contributor.authorThuita, M.
dc.contributor.authorMasso, C.
dc.contributor.authorMwirichia, R.
dc.date.accessioned2022-12-02T14:05:25Z
dc.date.available2022-12-02T14:05:25Z
dc.date.issued2022
dc.identifier.citationWachira, J.M., Kiplimo, D., Thuita, M., Masso, C. & Mwirichia, R. (2022). Community structure of nitrifying and denitrifying bacteria from effluents discharged into Lake Victoria, Kenya. Current Microbiology, 79(9):252, 1-10.
dc.identifier.issn0343-8651
dc.identifier.urihttps://hdl.handle.net/20.500.12478/7956
dc.description.abstractAn active microbial community of nitrifying and denitrifying bacteria is needed for efficient utilization of nitrogenous compounds from wastewater. In this study, we explored the bacterial community diversity and structure within rivers, treated and untreated wastewater treatment plants (WWTPs) discharging into Lake Victoria. Water samples were collected from rivers and WWTPs that drain into Lake Victoria. Physicochemical analysis was done to determine the level of nutrients or pollutant loading in the samples. Total community DNA was extracted, followed by Illumina high throughput sequencing to determine the total microbial community and abundance. Enrichment and isolation were then done to recover potential nitrifiers and denitrifiers. Physicochemical analysis pointed to high levels total nitrogen and ammonia in both treated and untreated WWTPs as compared to the samples from the lake and rivers. A total of 1,763 operational taxonomic units (OTUs) spread across 26 bacterial phyla were observed with the most dominant phylum being Proteobacteria. We observed a decreasing trend in diversity from the lake, rivers to WWTPs. The genus Planktothrix constituted 19% of the sequence reads in sample J2 collected from the lagoon. All the isolates recovered in this study were affiliated to three genera: Pseudomonas, Klebsiella and Enterobacter in the phylum Proteobacteria. A combination of metagenomic analysis and a culture-dependent approach helped us understand the relative abundance as well as potential nitrifiers and denitrifiers present in different samples. The recovered isolates could be used for in situ removal of nitrogenous compounds from contaminated wastewater.
dc.description.sponsorshipInternational Institute of Tropical Agriculture
dc.description.sponsorshipNational Research Fund, Kenya
dc.description.sponsorshipThe Alexander von Humboldt Equipment Grant
dc.format.extent1-10
dc.language.isoen
dc.subjectCommunity
dc.subjectBacterial Diseases
dc.subjectKenya
dc.titleCommunity structure of nitrifying and denitrifying bacteria from effluents discharged into Lake Victoria, Kenya
dc.typeJournal Article
cg.contributor.affiliationUniversity of Embu
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.coverage.regionAfrica
cg.coverage.regionEast Africa
cg.coverage.countryKenya
cg.coverage.hubCentral Africa Hub
cg.researchthemeNatural Resource Management
cg.identifier.bibtexciteidWACHIRA:2022a
cg.isijournalISI Journal
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectAgronomy
cg.iitasubjectBiodiversity
cg.iitasubjectDisease Control
cg.iitasubjectSoil Fertility
cg.journalCurrent Microbiology
cg.notesOpen Access Article; Published online: 14 Jul 2022
cg.accessibilitystatusOpen Access
cg.reviewstatusPeer Review
cg.usagerightslicenseCreative Commons Attribution 4.0 (CC BY 0.0)
cg.targetaudienceScientists
cg.identifier.doihttps://dx.doi.org/10.1007/s00284-022-02950-1
cg.iitaauthor.identifierMoses Thuita: 0000-0002-6731-9492
cg.iitaauthor.identifierCargele Masso: 0000-0002-3980-6832
cg.futureupdate.requiredNo
cg.identifier.issue9
cg.identifier.volume79


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