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    Validation of KASP markers associated with cassava mosaic disease resistance, storage root dry matter and provitamin A carotenoid contents in Ugandan cassava germplasm

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    Journal Article (2.295Mb)
    Date
    2022-11-23
    Author
    Esuma, W.
    Eyoo, O.
    Gwandu, F.
    Mukasa, S.
    Alicai, T.
    Ozimati, A.
    Nuwamanya, E.
    Rabbi, I.Y.
    Kawuki, R.
    Type
    Journal Article
    Review Status
    Peer Review
    Target Audience
    Scientists
    Metadata
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    Abstract/Description
    Introduction The intrinsic high heterozygosity of cassava makes conventional breeding ineffective for rapid genetic improvement. However, recent advances in next-generation sequencing technologies have enabled the use of high-density markers for genome-wide association studies, aimed at identifying single nucleotide polymorphisms (SNPs) linked to major traits such as cassava mosaic disease (CMD) resistance, dry matter content (DMC) and total carotenoids content (TCC). A number of these trait-linked SNPs have been converted to Kompetitive allele-specific polymerase chain reaction (KASP) markers for downstream application of marker assisted selection. Methods We assayed 13 KASP markers to evaluate their effectiveness in selecting for CMD, DMC and TCC in 1,677 diverse cassava genotypes representing two independent breeding populations in Uganda. Results Five KASP markers had significant co-segregation with phenotypes; CMD resistance (2), DMC (1) and TCC (2), with each marker accounting for at least 30% of the phenotypic variation. Markers located within the chromosomal regions for which strong marker-trait association loci have been characterised (chromosome 12 markers for CMD, chromosome 1 markers for DMC and TCC) had consistently superior ability to discriminate the respective phenotypes. Discussion The results indicate varying discriminatory abilities of the KASP markers assayed and the need for their context-based use for MAS, with PSY2_572 particularly effective in selecting for high TCC. Availing the effective KASP markers on cost-effective genotyping platforms could facilitate practical implementation of marker-assisted cassava breeding for accelerated genetic gains for CMD, DMC and provitamin A carotenoids.
    Acknowledgements
    We thank IR (IITA, Ibadan) for providing detailed technical information on the 13 KASP markers.
    https://dx.doi.org/10.3389/fpls.2022.1017275
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/8010
    IITA Authors ORCID
    Ismail Rabbihttps://orcid.org/0000-0001-9966-2941
    Digital Object Identifier (DOI)
    https://dx.doi.org/10.3389/fpls.2022.1017275
    Research Themes
    Biotech and Plant Breeding
    IITA Subjects
    Agronomy; Cassava; Food Security; Plant Breeding; Plant Production
    Agrovoc Terms
    Genetic Gain; Cassava; Manihot Esculenta; Genetic Markers; Genotypes; Uganda
    Regions
    Africa; East Africa
    Countries
    Uganda
    Hubs
    Headquarters and Western Africa Hub
    Journals
    Frontiers in Plant Science
    Collections
    • Journal and Journal Articles4842
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