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Evaluating the performance of lateral flow devices for total aflatoxins with special emphasis on their robustness under sub-Saharan conditions

dc.contributor.authorCvak, B.
dc.contributor.authorWarth, B.
dc.contributor.authorAtehnkeng, J.
dc.contributor.authorParich, A.
dc.contributor.authorMoritz, A.
dc.contributor.authorSulyok, M.
dc.contributor.authorKrska, R.
dc.date.accessioned2023-04-17T08:28:45Z
dc.date.available2023-04-17T08:28:45Z
dc.date.issued2021-10-20
dc.identifier.citationCvak, B., Warth, B., Atehnkeng, J., Parich, A., Moritz, A., Sulyok, M. & Krska, R. (2021). Evaluating the performance of lateral flow devices for total aflatoxins with special emphasis on their robustness under sub-Saharan conditions. Toxins, 13(11), 1-16.
dc.identifier.issn2072-6651
dc.identifier.urihttps://hdl.handle.net/20.500.12478/8134
dc.description.abstractAs aflatoxins are a global risk for humans and animals, testing methods for rapid on-site screening are increasingly needed alongside the standard analytical laboratory tools. In the presented study, lateral flow devices (LFDs) for rapid total aflatoxin screening were thoroughly investigated with respect to their matrix effects, cross-reactivity, their performance under harsh conditions in Sub-Saharan Africa (SSA), and their stability, as well as when compared with liquid chromatography-tandem mass spectrometry (LC-MS/MS). To analyze the matrix effects, qualitative test kits offering a certain cutoff level were used to screen different nut samples. In addition, these tests were challenged on their cross-reactivity with 230 fungal toxins and metabolites. Furthermore, the resulting measurements performed under harsh tropical conditions (up to 38.4 °C and 91% relative humidity) in SSA, specifically Burkina Faso and Mozambique, were compared with the results from a well-established and validated LC-MS/MS-based reference method. The comparison of the on-site LFD results with the reference method showed a good agreement: 86.4% agreement, 11.8% non-agreement, and 1.8% invalid test results. To test the robustness of the cutoff tests, short- and long-term stability testing was carried out in Mozambique and Nigeria. For both experiments, no loss of test performance could be determined. Finally, a subset of African corn samples was shipped to Austria and analyzed under laboratory conditions using semiquantitative aflatoxin tests. A good correlation was found between the rapid strip tests and the LC-MS/MS reference method. Overall, the evaluated LFDs showed satisfying results regarding their cross-reactivity, matrix effects, stability, and robustness.
dc.description.sponsorshipUniversity of Natural Resources and Life Sciences, Vienna
dc.description.sponsorshipRomer Labs Division Holding GmbH, Austria
dc.description.sponsorshipUniversity of Vienna
dc.description.sponsorshipInternational Institute of Tropical Agriculture
dc.description.sponsorshipEV Group E. Thallner GmbH, Austria
dc.description.sponsorshipQueen’s University Belfast
dc.format.extent1-16
dc.language.isoen
dc.subjectAflatoxins
dc.subjectToxins
dc.subjectScreening
dc.subjectMycotoxins
dc.subjectGroundnuts
dc.subjectDiseases
dc.subjectTests
dc.titleEvaluating the performance of lateral flow devices for total aflatoxins with special emphasis on their robustness under sub-Saharan conditions
dc.typeJournal Article
cg.contributor.crpAgriculture for Nutrition and Health
cg.contributor.affiliationUniversity of Natural Resources and Life Sciences, Austria
cg.contributor.affiliationRomer Labs Division Holding GmbH, Austria
cg.contributor.affiliationUniversity of Vienna
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationEV Group E. Thallner GmbH, Austria
cg.contributor.affiliationQueen’s University Belfast
cg.coverage.regionAfrica
cg.coverage.regionSouthern Africa
cg.coverage.regionWest Africa
cg.coverage.countryBurkina Faso (Upper Volta)
cg.coverage.countryMozambique
cg.coverage.countryNigeria
cg.coverage.hubCentral Africa Hub
cg.researchthemePlant Production and Health
cg.identifier.bibtexciteidCVAK:2021
cg.isijournalISI Journal
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectAflatoxin
cg.iitasubjectDisease Control
cg.iitasubjectFood Security
cg.iitasubjectGrain Legumes
cg.iitasubjectImpact Assessment
cg.iitasubjectPlant Diseases
cg.iitasubjectPlant Production
cg.journalToxins
cg.notesOpen Access Journal; Published online: 20 Oct 2021
cg.accessibilitystatusOpen Access
cg.reviewstatusPeer Review
cg.usagerightslicenseCreative Commons Attribution 4.0 (CC BY 0.0)
cg.targetaudienceScientists
cg.identifier.doihttps://doi.org/10.3390/toxins13110742
cg.futureupdate.requiredNo
cg.identifier.issue11
cg.identifier.volume13


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