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    Transcriptome and metabolome profiling identify factors potentially involved in pro-vitamin A accumulation in cassava landraces

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    Journal Article (7.552Mb)
    Date
    2023-06
    Author
    Olayide, P.
    Alexandersson, E.
    Tzfadia, O.
    Lenman, M.
    Gisel, A.
    Stavolone, L.
    Type
    Journal Article
    Review Status
    Peer Review
    Target Audience
    Scientists
    Metadata
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    Abstract/Description
    Cassava (Manihot esculenta Crantz) is a predominant food security crop in several developing countries. Its storage roots, rich in carbohydrate, are deficient in essential micronutrients, including provitamin A carotenoids. Increasing carotenoid content in cassava storage roots is important to reduce the incidence of vitamin A deficiency, a public health problem in sub-Saharan Africa. However, cassava improvement advances slowly, mainly due to limited information on the molecular factors influencing β-carotene accumulation in cassava. To address this problem, we performed comparative transcriptomic and untargeted metabolic analyses of roots and leaves of eleven African cassava landraces ranging from white to deep yellow colour, to uncover regulators of carotenoid biosynthesis and accumulation with conserved function in yellow cassava roots. Sequence analysis confirmed the presence of a mutation, known to influence β-carotene content, in PSY transcripts of deep yellow but not of pale yellow genotypes. We identified genes and metabolites with expression and accumulation levels significantly associated with β-carotene content. Particularly an increased activity of the abscisic acid catabolism pathway together with a reduced amount of L-carnitine, may be related to the carotenoid pathway flux, higher in yellow than in white storage roots. In fact, NCED_3.1 was specifically expressed at a lower level in all yellow genotypes suggesting that it could be a potential target for increasing carotenoid accumulation in cassava. These results expand the knowledge on metabolite compositions and molecular mechanisms influencing carotenoid biosynthesis and accumulation in cassava and provide novel information for biotechnological applications and genetic improvement of cassava with high nutritional values.
    https://doi.org/10.1016/j.plaphy.2023.107713
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/8177
    IITA Authors ORCID
    ANDREAS GISELhttps://orcid.org/0000-0001-7218-9488
    LIVIA STAVOLONEhttps://orcid.org/0000-0002-0691-1302
    Digital Object Identifier (DOI)
    https://doi.org/10.1016/j.plaphy.2023.107713
    Research Themes
    Biotech and Plant Breeding
    IITA Subjects
    Agronomy; Cassava; Food Security; Plant Breeding; Plant Production
    Agrovoc Terms
    Cassava; Carotenoids; Transcriptome; Metabolites; Food Security; Sub-Saharan Africa
    Regions
    Africa; West Africa
    Countries
    Nigeria
    Hubs
    Headquarters and Western Africa Hub
    Journals
    Plant Physiology and Biochemistry
    Collections
    • Journal and Journal Articles4943
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