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Endophytic fungi improve management of the burrowing nematode in banana (Musa spp.) through enhanced expression of defence-related genes

dc.contributor.authorKisaakye, J.
dc.contributor.authorFourie, H.
dc.contributor.authorCoyne, D.
dc.contributor.authorCortada, L.
dc.contributor.authorKhamis, F.M.
dc.contributor.authorSubramanian, S.
dc.contributor.authorMasinde, S.
dc.contributor.authorHaukeland, S.
dc.date.accessioned2023-08-01T09:54:03Z
dc.date.available2023-08-01T09:54:03Z
dc.date.issued2023-03-02
dc.identifier.citationKisaakye, J., Fourie, H., Coyne, D., Cortada, L., Khamis, F.M., Subramanian, S., ... & Haukeland, S. (2023). Endophytic fungi improve management of the burrowing nematode in banana (Musa spp.) through enhanced expression of defence-related genes. Nematology, 25(4), 427-442.
dc.identifier.issn1388-5545
dc.identifier.urihttps://hdl.handle.net/20.500.12478/8231
dc.description.abstractThe root-burrowing nematode, Radopholus similis, is reputedly the most damaging nematode pest of banana and responsible for major production losses. In this study, the endophytic potential of 13 fungal isolates was assessed for the management of R. similis in East African Highland bananas (‘Ng’ombe’). All isolates successfully colonised tissue-cultured banana roots, with isolates from Trichoderma, Fusarium and Hypocrea producing the highest (⩾49.1%) and Beauveria isolates the lowest (⩽14.4%) colonisation. The fungal endophytes T. asperellum (ICIPE 700) and H. lixii (ICIPE 697) were the most effective in reducing R. similis densities (>81%) relative to the non-inoculated control. However, the combined inoculation of ICIPE 700 and ICIPE 697 led to greater suppression of R. similis (>21%) relative to individual inoculation. Suppression of R. similis following inoculation of banana roots with ICIPE 700 and/or ICIPE 697 was associated with the significant upregulation of the defence-related gene PR-1, the cell signalling gene calmodulin Ca2+ and the cell-wall-strengthening gene β-1,3-glucan synthase. This study demonstrates the potential for nematode management in bananas with fungal endophytes, especially using the isolates ICIPE 700 and ICIPE 697 when combined.
dc.description.sponsorshipH2020 European Union
dc.description.sponsorshipUK’s Foreign, Commonwealth and Development Office
dc.description.sponsorshipSwedish International Development Cooperation Agency
dc.description.sponsorshipSwiss Agency for Development and Cooperation
dc.description.sponsorshipFederal Democratic Republic of Ethiopia
dc.description.sponsorshipGovernment of the Republic of Kenya
dc.format.extent427-442
dc.language.isoen
dc.subjectBiological Control
dc.subjectEndophytes
dc.subjectGene Expression
dc.subjectRadopholus Similis
dc.subjectTissue Culture
dc.subjectBananas
dc.titleEndophytic fungi improve management of the burrowing nematode in banana (Musa spp.) through enhanced expression of defence-related genes
dc.typeJournal Article
cg.contributor.crpRoots, Tubers and Bananas
cg.contributor.affiliationInternational Centre of Insect Physiology and Ecology
cg.contributor.affiliationNorth-West University, South Africa
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationGhent University
cg.contributor.affiliationNorwegian Institute for Bioeconomy Research
cg.coverage.regionAfrica
cg.coverage.regionEast Africa
cg.coverage.countryKenya
cg.coverage.hubEastern Africa Hub
cg.researchthemeNatural Resource Management
cg.identifier.bibtexciteidKISAAKYE:2023
cg.isijournalISI Journal
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectAgronomy
cg.iitasubjectBanana
cg.iitasubjectDisease Control
cg.iitasubjectFood Security
cg.iitasubjectPlant Breeding
cg.iitasubjectPlant Diseases
cg.iitasubjectPlant Health
cg.iitasubjectPlant Production
cg.journalNematology
cg.notesOpen Access Article; Published online: 02 Mar 2023
cg.accessibilitystatusOpen Access
cg.reviewstatusPeer Review
cg.usagerightslicenseCreative Commons Attribution 4.0 (CC BY 0.0)
cg.targetaudienceScientists
cg.identifier.doihttps://doi.org/10.1163/15685411-bja10229
cg.iitaauthor.identifierDaniel Coyne: 0000-0002-2030-6328
cg.futureupdate.requiredNo
cg.identifier.issue4
cg.identifier.volume25


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