The capability of winged bean to support food and nutrition security in sub-Saharan Africa is recurrently being affected by several constraints, which include a lack of genetic improvement. The dearth of adequate information on the level of available genetic diversity in winged bean germplasm has been a major setback in planning appropriate improvement programs. Fifteen winged bean accessions were assessed for genetic diversity using 10 quantitative traits and 10 simple sequence repeat (SSR) markers. The accessions were laid out in RCBD with three replicates for two growing seasons. Leaf samples were obtained from 10 plants representing each accession for SSR marker genotyping. The accessions exhibited significant (p < 0.05) differences for measured traits. Broad-sense heritability estimates varied from 10.31% for days to first plant maturity to 72.67% for pod weight. Pod weight had a positive and significant correlations with pod length (0.53, p < 0.05), pod width (0.70, p < 0.01), and number of seeds per pod (0.64, p < 0.01). However, the number of seeds per pod was negatively correlated with days to maturity (−0.71, p < 0.01). Number of seeds per pod was positively predicted by pod weight, seed thickness, and days to maturity. Cluster analysis delineated the accessions into two distinct groups. Average number of alleles of 4.2, gene diversity of 0.25, and polymorphic information content of 0.22 were recorded. Analysis of molecular variance revealed intra-accession variation of 95% as compared to inter-accession variation of 5%. Two primary genetic groups were identified and only three accessions, namely TPt-6, TPt-126, and TPt-48, showed genetic purity. The results of this study provide the basis for exploiting the existing diversity for winged bean improvement.

https://doi.org/10.3390/agronomy13082150

Multi standard citation

APAHarvardMLAVancouverChicagoIEEE

Temitope Shondehttps://orcid.org/0000-0003-0469-2211

Ayomide Ajoke Bhadmushttps://orcid.org/0000-0002-3621-8082

Olaniyi Oyatomihttps://orcid.org/0000-0003-3094-374X

Michael Abbertonhttps://orcid.org/0000-0003-2555-9591

https://doi.org/10.3390/agronomy13082150

Biotech and Plant Breeding

Agronomy; Food Security; Genetic Improvement; Plant Genetic Resources

Accessions; Genetic Diversity; Psophocarpus Tetragonolobus; Winged Bean; Gene Banks

Africa; West Africa

Nigeria

Headquarters and Western Africa Hub

Agronomy