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Efficient sugar utilization and transition from oxidative to substrate-level phosphorylation in high starch storage roots of African cassava genotypes

dc.contributor.authorLamm, C.E.
dc.contributor.authorRabbi, I.Y.
dc.contributor.authorMedeiros, D.B.
dc.contributor.authorRosado-Souza, L.
dc.contributor.authorPommerrenig, B.
dc.contributor.authorDahmani, I.
dc.contributor.authorRuscher, D.
dc.contributor.authorHofmann, J.
dc.contributor.authorvan Doorn, A.M.
dc.contributor.authorSchlereth, A.
dc.contributor.authorNeuhaus, H.E.
dc.contributor.authorFernie, A.R.
dc.contributor.authorSonnewald, U.
dc.contributor.authorZierer, W.
dc.date.accessioned2023-12-11T11:27:09Z
dc.date.available2023-12-11T11:27:09Z
dc.date.issued2023-06-17
dc.identifier.citationLamm, C.E., Rabbi, I.Y., Medeiros, D.B., Rosado‐Souza, L., Pommerrenig, B., Dahmani, I., ... & Zierer, W. (2023). Efficient sugar utilization and transition from oxidative to substrate‐level phosphorylation in high starch storage roots of African cassava genotypes. Plant Journal, 116(1), 38-57.
dc.identifier.issn0960-7412
dc.identifier.urihttps://hdl.handle.net/20.500.12478/8349
dc.description.abstractCassava's storage roots represent one of the most important sources of nutritional carbohydrates worldwide. Particularly, smallholder farmers in sub-Saharan Africa depend on this crop plant, where resilient and yield-improved varieties are of vital importance to support steadily increasing populations. Aided by a growing understanding of the plant's metabolism and physiology, targeted improvement concepts already led to visible gains in recent years. To expand our knowledge and to contribute to these successes, we investigated storage roots of eight cassava genotypes with differential dry matter content from three successive field trials for their proteomic and metabolic profiles. At large, the metabolic focus in storage roots transitioned from cellular growth processes toward carbohydrate and nitrogen storage with increasing dry matter content. This is reflected in higher abundance of proteins related to nucleotide synthesis, protein turnover, and vacuolar energization in low starch genotypes, while proteins involved in sugar conversion and glycolysis were more prevalent in high dry matter genotypes. This shift in metabolic orientation was underlined by a clear transition from oxidative- to substrate-level phosphorylation in high dry matter genotypes. Our analyses highlight metabolic patterns that are consistently and quantitatively associated with high dry matter accumulation in cassava storage roots, providing fundamental understanding of cassava's metabolism as well as a data resource for targeted genetic improvement.
dc.description.sponsorshipBill & Melinda Gates Foundation
dc.format.extent38-57
dc.language.isoen
dc.subjectManihot Esculenta
dc.subjectStarch
dc.subjectEnergy
dc.subjectGlycolysis
dc.subjectOxidative Phosphorylation
dc.subjectProteomics
dc.subjectMetabolomics
dc.titleEfficient sugar utilization and transition from oxidative to substrate-level phosphorylation in high starch storage roots of African cassava genotypes
dc.typeJournal Article
cg.contributor.crpRoots, Tubers and Bananas
cg.contributor.affiliationFriedrich-Alexander-Universitat Erlangen-Nurnberg
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationMax Planck Institute of Molecular Plant Physiology
cg.contributor.affiliationUniversity of Kaiserslautern
cg.coverage.regionACP
cg.coverage.regionAfrica
cg.coverage.regionEurope
cg.coverage.regionWest Africa
cg.coverage.countryGermany
cg.coverage.countryNigeria
cg.coverage.hubHeadquarters and Western Africa Hub
cg.researchthemeBiotech and Plant Breeding
cg.identifier.bibtexciteidLAMM:2023
cg.isijournalISI Journal
cg.authorship.typesCGIAR and advanced research institute
cg.iitasubjectAgronomy
cg.iitasubjectCassava
cg.iitasubjectFood Security
cg.iitasubjectPlant Breeding
cg.iitasubjectPlant Production
cg.iitasubjectSmallholder Farmers
cg.iitasubjectValue Chains
cg.journalPlant Journal
cg.notesOpen Access Article
cg.accessibilitystatusOpen Access
cg.reviewstatusPeer Review
cg.usagerightslicenseCreative Commons Attribution 4.0 (CC BY 0.0)
cg.targetaudienceScientists
cg.identifier.doihttps://doi.org/10.1111/tpj.16357
cg.iitaauthor.identifierIsmail Rabbi: 0000-0001-9966-2941
cg.futureupdate.requiredNo
cg.identifier.issue1
cg.identifier.volume116


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