The primary goal of this study was to compare the multiplication rates of yam varieties propagated through organogenesis and somatic embryogenesis (SE). Callus was induced from axillary bud explants of three genotypes of Dioscorea rotundata (Asiedu, Ekiti2a, and Kpamyo) and two genotypes of Dioscorea alata (Swaswa and TDa2014) cultured in Murashige and Skoog (MS) medium containing 9.1 µM 2,4-dichlorophenoxylacetic acid and 5.4 µM naphthaleneacetic acid. Plantlets were regenerated in MS containing 4.4 µM benzylaminopurine and 34 µM uniconazole-P through SE. Single-node cuttings of the five genotypes were grown in MS for 8 wk via organogenesis. The SE and organogenesis regenerants were acclimatized and potted in a 2 (propagation techniques (PTs)) × 5 (genotypes) factorial arranged in a completely randomized design (r = 10). The multiplication ratios (MR), number of tubers (NoT) of the SE, and organogenesis regenerants were collected and analyzed using ANOVA, and means were separated using DMRT (P ≤ 0.05). The SE and organogenesis MR ranged from 1:2 (TDa2014) to 1:8 (Asiedu) and 1:4 (Asiedu) to 1:5 (Ekiti2a and TDa2014), respectively. The NoT differed among genotypes, ranging from 1.15 ± 0.49 (Swaswa) to 2.45 ± 1.39 (Asiedu), and between PTs, ranging from 1.42 ± 0.70 (SE) to 1.86 ± 1.11 (organogenesis). The optimum propagation pathway was genotype-specific.

https://doi.org/10.1007/s11627-023-10397-7

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CHUKWUNALU OKOLIEhttps://orcid.org/0000-0002-1924-5855

Morufat Balogunhttps://orcid.org/0000-0001-8770-5529

Norbert Maroyahttps://orcid.org/0000-0002-7079-4729

https://doi.org/10.1007/s11627-023-10397-7

Biotech and Plant Breeding

Agronomy; Food Security; Genetic Improvement; Plant Breeding; Plant Production; Yam

Yams; Seed Systems; Varieties; Organogenesis; Somatic Embryogenesis; Seedlings

Africa; West Africa

Nigeria

Headquarters and Western Africa Hub

In Vitro Cellular & Developmental Biology-Plant