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Assessing the carotenoid profiles and allelic diversity of yellow maize inbred lines adapted to mid-altitude subhumid maize agroecology in Ethiopia

dc.contributor.authorGaroma, B.
dc.contributor.authorAzimach, G.
dc.contributor.authorBante, K.
dc.contributor.authorMenkir, A.
dc.date.accessioned2024-09-23T07:21:29Z
dc.date.available2024-09-23T07:21:29Z
dc.date.issued2024-07-23
dc.identifier.citationGaroma, B., Azimach, G., Bante, K. & Menkir, A. (2024). Assessing the carotenoid profiles and allelic diversity of yellow maize inbred lines adapted to mid-altitude subhumid maize agroecology in Ethiopia. Frontiers in Plant Science, 15: 1406550, 1-9.
dc.identifier.issn1664-462X
dc.identifier.urihttps://hdl.handle.net/20.500.12478/8547
dc.description.abstractBiofortification of provitamin A in maize is an attractive and sustainable remedy to the problem of vitamin A deficiency in developing countries. The utilization of molecular markers represents a promising avenue to facilitate the development of provitamin A (PVA)-enriched maize varieties. We screened 752 diverse tropical yellow/orange maize lines using kompetitive allele-specific PCR (KASP) makers to validate the use of KASP markers in PVA maize breeding. To this end, a total of 161 yellow/orange inbred lines, selected from among the 752 lines, were evaluated for their endosperm PVA and other carotenoid compounds levels in two separate trials composed of 63 and 98 inbred lines in 2020 and 2021, respectively. Significant differences (p < 0.001) were observed among the yellow maize inbred lines studied for all carotenoid profiles. An inbred line TZMI1017, introduced by the International Institute of Tropical Agriculture (IITA) showed the highest level of PVA (12.99 µg/g) and β-carotene (12.08 µg/g). The molecular screening showed 43 yellow maize inbred lines carrying at least three of the favorable alleles of the KASP markers. TZMI1017 inbred line also carried the favorable alleles of almost all markers. In addition, nine locally developed inbred lines had medium to high PVA concentrations varying from 5.11 µg/g to 10.76 µg/g and harbored the favorable alleles of all the KASP PVA markers. Association analysis between molecular markers and PVA content variation in the yellow/orange maize inbred lines did not reveal a significant, predictable correlation. Further investigation is warranted to elucidate the underlying genetic architecture of the PVA content in this germplasm. However, we recommend strategic utilization of the maize-inbred lines with higher PVA content to enhance the PVA profile of the breeding program’s germplasm.
dc.description.sponsorshipHarvestPlus
dc.format.extent1-9
dc.language.isoen
dc.subjectBiofortification
dc.subjectCarotenoids
dc.subjectProvitamins
dc.subjectAlleles
dc.subjectMarker-Assisted Selection
dc.subjectMaize
dc.subjectInbred Lines
dc.titleAssessing the carotenoid profiles and allelic diversity of yellow maize inbred lines adapted to mid-altitude subhumid maize agroecology in Ethiopia
dc.typeJournal Article
cg.contributor.crpMaize
cg.contributor.affiliationBako National Maize Research Center, Ethiopia
cg.contributor.affiliationEthioAgri-CEFT, Ethiopia
cg.contributor.affiliationJima University
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.coverage.regionAfrica
cg.coverage.regionEast Africa
cg.coverage.countryEthiopia
cg.coverage.hubHeadquarters and Western Africa Hub
cg.researchthemeBiotech and Plant Breeding
cg.identifier.bibtexciteidGAROMA:2024
cg.isijournalISI Journal
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectAgronomy
cg.iitasubjectBiofortification
cg.iitasubjectFood Security
cg.iitasubjectMaize
cg.iitasubjectPlant Breeding
cg.iitasubjectPlant Production
cg.journalFrontiers in Plant Science
cg.notesOpen Access Journal
cg.accessibilitystatusOpen Access
cg.reviewstatusPeer Review
cg.usagerightslicenseCreative Commons Attribution 4.0 (CC BY 0.0)
cg.targetaudienceScientists
cg.identifier.doihttps://doi.org/10.3389/fpls.2024.140655
cg.iitaauthor.identifierAbebe Menkir: 0000-0002-5907-9177
cg.futureupdate.requiredNo
cg.identifier.issue1406550
cg.identifier.volume15


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