• Contact Us
    • Send Feedback
    • Login
    View Item 
    •   Home
    • Journal and Journal Articles
    • Journal and Journal Articles
    • View Item
    •   Home
    • Journal and Journal Articles
    • Journal and Journal Articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    Whole Repository
    CollectionsIssue DateRegionCountryHubAffiliationAuthorsTitlesSubject
    This Sub-collection
    Issue DateRegionCountryHubAffiliationAuthorsTitlesSubject

    My Account

    Login

    Welcome to the International Institute of Tropical Agriculture Research Repository

    What would you like to view today?

    DNA barcoding of the main cultivated yams and selected wild species in the genus Dioscorea

    Thumbnail
    View/Open
    S16ArtGirmaBarcodingInthomNodev.pdf (385.7Kb)
    Date
    2016-05
    Author
    Girma, G.
    Spillane, Charles
    Gedil, M.
    Type
    Journal Article
    Target Audience
    Scientists
    Metadata
    Show full item record
    Abstract/Description
    Distinguishing yam species based on morphological traits is extremely difficult and unreliable, posing a challenge to breeders and genebank curators. Development of a molecular assay based on DNA barcoding can facilitate rapid and accurate identification of important Dioscorea species. To develop a DNA barcoding system for Dioscorea species identification, the rbcL and matK loci (in unison and in combination), the non-coding intergenic spacer trnH-psbA of the chloroplast genome, and the nuclear ITS regions were investigated using criteria for developing candidate DNA barcodes. All DNA barcoding sequences were assessed for ease of PCR amplification, sequence quality and species discriminatory power. Amongst the markers investigated, the matK locus performed well in terms of species identification (63.2%), in addition to detecting high interspecific variation with mean divergence of 0.0196 (SD=0.0209). The combination of the two coding regions (rbcL + matK) was determined to be the optimal (76.2%) DNA barcoding approach as 16 out of 21 species could be defined. While the rbcL exhibited good PCR amplification efficiency and sequence quality, its species discriminatory power was relatively poor with 47.6% identification. Similarly, the trnH-psbA region had a weak discrimination efficiency of only 36.8%. While the development of more robust DNA barcoding systems is an ongoing challenge, our results indicate that the rbcL + matK combination can be utilized as multi-locus DNA barcode regions for Dioscorea species identification.
    https://dx.doi.org/10.1111/jse.12183
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/862
    Digital Object Identifier (DOI)
    https://dx.doi.org/10.1111/jse.12183
    IITA Subjects
    Yam
    Agrovoc Terms
    Dioscorea; Dna; Yams; Germplasm; Gene Banks
    Regions
    Africa; West Africa
    Countries
    Nigeria
    Journals
    Journal of Systematics and Evolution
    Collections
    • Journal and Journal Articles4835
    copyright © 2019  IITASpace. All rights reserved.
    IITA | Open Access Repository