• Contact Us
    • Send Feedback
    • Login
    View Item 
    •   Home
    • Journal and Journal Articles
    • Journal and Journal Articles
    • View Item
    •   Home
    • Journal and Journal Articles
    • Journal and Journal Articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    Whole Repository
    CollectionsIssue DateRegionCountryHubAffiliationAuthorsTitlesSubject
    This Sub-collection
    Issue DateRegionCountryHubAffiliationAuthorsTitlesSubject

    My Account

    Login

    Welcome to the International Institute of Tropical Agriculture Research Repository

    What would you like to view today?

    Incidence, distribution and first identification of citrus tristeza virus by RTPCR in citrus orchards in southWestern Nigeria

    Thumbnail
    Date
    2015
    Author
    Adediji, A.O.
    Atiri, G.I.
    Kumar, P.L.
    Type
    Journal Article
    Metadata
    Show full item record
    Abstract/Description
    In order to ascertain the presence, incidence, and distribution of Citrus tristeza virus (CTV), extensive surveys were conducted in citrus growing areas of south-western Nigeria during 2011-12. A total of 565 citrus trees were sampled in three states. Leaf samples were collected and symptom intensity of the trees was recorded. Symptoms observed included stem pitting, stunting, vein clearing and leaf curling. Nucleic acid extracts from leaves were used to detect CTV by RT-PCR using primers PIN1 and PIN2 directed to the conserved 3’ untranslated region. Results show the presence of CTV in the three states surveyed while symptom severity differed from one location to another. Disease incidence varied from 85.2% in Oyo State to 48.4% in Ogun State. Citrus trees were infected regardless of scionrootstock combinations and the virus was detected in sweet orange (87.0%), tangerine (77.6%), grapefruit (73.8%) and lemon (69.5%). This is the first report of molecular detection of CTV in Nigeria.In order to ascertain the presence, incidence, and distribution of Citrus tristeza virus (CTV), extensive surveys were conducted in citrus growing areas of south-western Nigeria during 2011-12. A total of 565 citrus trees were sampled in three states. Leaf samples were collected and symptom intensity of the trees was recorded. Symptoms observed included stem pitting, stunting, vein clearing and leaf curling. Nucleic acid extracts from leaves were used to detect CTV by RT-PCR using primers PIN1 and PIN2 directed to the conserved 3’ untranslated region. Results show the presence of CTV in the three states surveyed while symptom severity differed from one location to another. Disease incidence varied from 85.2% in Oyo State to 48.4% in Ogun State. Citrus trees were infected regardless of scionrootstock combinations and the virus was detected in sweet orange (87.0%), tangerine (77.6%), grapefruit (73.8%) and lemon (69.5%). This is the first report of molecular detection of CTV in Nigeria.
    https://dx.doi.org/10.17660/ActaHortic.2015.1065.95
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/887
    Digital Object Identifier (DOI)
    https://dx.doi.org/10.17660/ActaHortic.2015.1065.95
    Agrovoc Terms
    Disease Surveys; Symptoms; Citrus
    Regions
    Africa; West Africa
    Countries
    Nigeria
    Journals
    Acta Horticulturae
    Collections
    • Journal and Journal Articles4835
    copyright © 2019  IITASpace. All rights reserved.
    IITA | Open Access Repository