Show simple item record

Developing Cassava Mosaic Disease resistant cassava varieties in Ghana using a marker assisted selection approach

dc.contributor.authorParkes, E.
dc.contributor.authorFregene, M.
dc.contributor.authorDixon, A.
dc.contributor.authorOkogbenin, Emmanuel
dc.contributor.authorBoakye-Peprah, B.
dc.contributor.authorLabuschagne, M.T.
dc.date.accessioned2019-12-04T10:58:02Z
dc.date.available2019-12-04T10:58:02Z
dc.date.issued2015
dc.identifier.citationParkes, E., Fregene, M., Dixon, A., Okogbenin, E., Boakye-Peprah, B., & Labuschagne, M. (2015). Developing cassava mosaic disease resistant cassava varieties in Ghana using a marker assisted selection approach. Euphytica, 203(3), 549-556.
dc.identifier.issn0014-2336
dc.identifier.urihttps://hdl.handle.net/20.500.12478/899
dc.description.abstractCassava is a major staple and food security crop in Africa which can produce a crop under uncertain rainfall and low fertility conditions. How- ever, it suffers from several pests and diseases, with Cassava Mosaic Disease (CMD) being the main biotic constraint in production in sub-Saharan Africa. It is caused by cassava mosaic geminiviruses. Plants infected with CMD usually produce a few or no storage roots. Markers associated with resistance genes to CMD have been mapped, including a major dominant gene, CMD2 . Eight CMD resistant geno- types from CIAT were used in crosses with three cassava landraces and one IITA breeding genotype. CMD evaluation was done on the parents and 525 F 1 progenies comprising of individuals pre-selected for CMD resistance based on average severity scores of 1 and 2. Multiple marker analysis was used to screen for the CMD2 gene, where four markers associated with this gene were used. Of the tested genotypes 83 % showed at least one marker allele for the CMD2 gene. Two CIAT genotypes (CR52A-31 and AR14-10) had all four marker alleles. TMEII had three alleles associated with CMD2 while Dabodabo had one marker allele. This suggests that Dababo may have alternative genes than CMD2 for CMD resistance. The F 1 families showed segregation for the CMD2 gene. A total of 88 individuals (17 %) had all four marker alleles associated with the CMD2 gene. A total of 179 individuals had between one to three marker alleles each associated with CMD2 . A total of 91 individuals had no marker allele associated with the CMD2 geneCassava is a major staple and food security crop in Africa which can produce a crop under uncertain rainfall and low fertility conditions. How- ever, it suffers from several pests and diseases, with Cassava Mosaic Disease (CMD) being the main biotic constraint in production in sub-Saharan Africa. It is caused by cassava mosaic geminiviruses. Plants infected with CMD usually produce a few or no storage roots. Markers associated with resistance genes to CMD have been mapped, including a major dominant gene, CMD2 . Eight CMD resistant geno- types from CIAT were used in crosses with three cassava landraces and one IITA breeding genotype. CMD evaluation was done on the parents and 525 F 1 progenies comprising of individuals pre-selected for CMD resistance based on average severity scores of 1 and 2. Multiple marker analysis was used to screen for the CMD2 gene, where four markers associated with this gene were used. Of the tested genotypes 83 % showed at least one marker allele for the CMD2 gene. Two CIAT genotypes (CR52A-31 and AR14-10) had all four marker alleles. TMEII had three alleles associated with CMD2 while Dabodabo had one marker allele. This suggests that Dababo may have alternative genes than CMD2 for CMD resistance. The F 1 families showed segregation for the CMD2 gene. A total of 88 individuals (17 %) had all four marker alleles associated with the CMD2 gene. A total of 179 individuals had between one to three marker alleles each associated with CMD2 . A total of 91 individuals had no marker allele associated with the CMD2 geneCassava is a major staple and food security crop in Africa which can produce a crop under uncertain rainfall and low fertility conditions. How- ever, it suffers from several pests and diseases, with Cassava Mosaic Disease (CMD) being the main biotic constraint in production in sub-Saharan Africa. It is caused by cassava mosaic geminiviruses. Plants infected with CMD usually produce a few or no storage roots. Markers associated with resistance genes to CMD have been mapped, including a major dominant gene, CMD2 . Eight CMD resistant geno- types from CIAT were used in crosses with three cassava landraces and one IITA breeding genotype. CMD evaluation was done on the parents and 525 F 1 progenies comprising of individuals pre-selected for CMD resistance based on average severity scores of 1 and 2. Multiple marker analysis was used to screen for the CMD2 gene, where four markers associated with this gene were used. Of the tested genotypes 83 % showed at least one marker allele for the CMD2 gene. Two CIAT genotypes (CR52A-31 and AR14-10) had all four marker alleles. TMEII had three alleles associated with CMD2 while Dabodabo had one marker allele. This suggests that Dababo may have alternative genes than CMD2 for CMD resistance. The F 1 families showed segregation for the CMD2 gene. A total of 88 individuals (17 %) had all four marker alleles associated with the CMD2 gene. A total of 179 individuals had between one to three marker alleles each associated with CMD2 . A total of 91 individuals had no marker allele associated with the CMD2 gene
dc.language.isoen
dc.subjectCassava
dc.subjectGeminiviruses
dc.subjectAfrican Cassava Mosaic Virus
dc.titleDeveloping Cassava Mosaic Disease resistant cassava varieties in Ghana using a marker assisted selection approach
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.crpRoots, Tubers and Bananas
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationMinistry of Agriculture, Nigeria
cg.contributor.affiliationDonald Danforth Plant Science Center
cg.contributor.affiliationSierra Leone Agricultural Research Institute
cg.contributor.affiliationNational Root Crops Research Institute, Nigeria
cg.contributor.affiliationCouncil for Scientific and Industrial Research, Ghana
cg.contributor.affiliationUniversity of the Free State
cg.coverage.regionAfrica
cg.coverage.regionWest Africa
cg.coverage.countryNigeria
cg.isijournalISI Journal
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectCassava
cg.journalEuphytica
cg.howpublishedFormally Published
cg.accessibilitystatusLimited Access
local.dspaceid76398
cg.identifier.doihttps://dx.doi.org/10.1007/s10681-014-1262-6


Files in this item

Thumbnail
Name:
S15ArtParkesDevelopingInthomDev.pdf
Size:
364.8Kb
Format:
PDF
View/Open

This item appears in the following Collection(s)

Show simple item record