| dc.contributor.author | Shitta, N.S. |
| dc.contributor.author | Abberton, M.T. |
| dc.contributor.author | Adesoye, A.I. |
| dc.contributor.author | Adewale, D.B. |
| dc.contributor.author | Oyatomi, O. |
| dc.date.accessioned | 2019-12-04T10:58:06Z |
| dc.date.available | 2019-12-04T10:58:06Z |
| dc.date.issued | 2015-03 |
| dc.identifier.citation | Shitta, N.S., Abberton, M.T., Adesoye, A.I., Adewale, D.B., & Oyatomi, O. (2015). Analysis of genetic diversity of African yam bean using SSR markers derived from cowpea. Plant Genetic Resources, 1-7. |
| dc.identifier.issn | 1479-2621 |
| dc.identifier.uri | https://hdl.handle.net/20.500.12478/915 |
| dc.description.abstract | African yam bean, AYB (Sphenostylis stenocarpa Hochst. ex. A. Rich Harms), is a tuberous
legume of tropical Africa. AYB has the potential to significantly boost food security due to
its considerable nutritional qualities. However, the crop is underutilized. To efficiently utilize
AYB genetic resources for its improvement, it is necessary to understand the crop’s diversity.
This study investigated the amplification ability of 36 cowpea simple sequence repeat (SSR)
primers across AYB genomic DNA, extracted from 67 accessions. Thirteen (36%) of the
cowpea SSRs showed transferability in AYB. Eight of these SSRs amplified above 60% of
AYB accessions and generated 55 polymorphic fragments with an average of 6.9 per primer.
Polymorphic information content ranged from 0.6691 to 0.8857 with an average of 0.7791.
This study also assessed the genetic diversity within 67 AYB accessions using eight cowpea
(Vigna unguiculata L. Walp)-derived SSR primers. The result revealed a high level of genetic
diversity with simple matching coefficient ranging from 0.458 to 1.000. A dendrogram depicting
three main clusters was generated based on unweighted pair group method with arithmetic
average. Cluster 1 was the most diverse with a dissimilarity range of 0.517–1.000. The level of
genetic diversity revealed in this study indicates that the studied AYB germplasm can be
exploited for genetic improvement. Additionally, the transferable markers will aid AYB
genome research and also make possible the comparative mapping between AYB and cowpea.African yam bean, AYB (Sphenostylis stenocarpa Hochst. ex. A. Rich Harms), is a tuberous
legume of tropical Africa. AYB has the potential to significantly boost food security due to
its considerable nutritional qualities. However, the crop is underutilized. To efficiently utilize
AYB genetic resources for its improvement, it is necessary to understand the crop’s diversity.
This study investigated the amplification ability of 36 cowpea simple sequence repeat (SSR)
primers across AYB genomic DNA, extracted from 67 accessions. Thirteen (36%) of the
cowpea SSRs showed transferability in AYB. Eight of these SSRs amplified above 60% of
AYB accessions and generated 55 polymorphic fragments with an average of 6.9 per primer.
Polymorphic information content ranged from 0.6691 to 0.8857 with an average of 0.7791.
This study also assessed the genetic diversity within 67 AYB accessions using eight cowpea
(Vigna unguiculata L. Walp)-derived SSR primers. The result revealed a high level of genetic
diversity with simple matching coefficient ranging from 0.458 to 1.000. A dendrogram depicting
three main clusters was generated based on unweighted pair group method with arithmetic
average. Cluster 1 was the most diverse with a dissimilarity range of 0.517–1.000. The level of
genetic diversity revealed in this study indicates that the studied AYB germplasm can be
exploited for genetic improvement. Additionally, the transferable markers will aid AYB
genome research and also make possible the comparative mapping between AYB and cowpea.African yam bean, AYB (Sphenostylis stenocarpa Hochst. ex. A. Rich Harms), is a tuberous
legume of tropical Africa. AYB has the potential to significantly boost food security due to
its considerable nutritional qualities. However, the crop is underutilized. To efficiently utilize
AYB genetic resources for its improvement, it is necessary to understand the crop’s diversity.
This study investigated the amplification ability of 36 cowpea simple sequence repeat (SSR)
primers across AYB genomic DNA, extracted from 67 accessions. Thirteen (36%) of the
cowpea SSRs showed transferability in AYB. Eight of these SSRs amplified above 60% of
AYB accessions and generated 55 polymorphic fragments with an average of 6.9 per primer.
Polymorphic information content ranged from 0.6691 to 0.8857 with an average of 0.7791.
This study also assessed the genetic diversity within 67 AYB accessions using eight cowpea
(Vigna unguiculata L. Walp)-derived SSR primers. The result revealed a high level of genetic
diversity with simple matching coefficient ranging from 0.458 to 1.000. A dendrogram depicting
three main clusters was generated based on unweighted pair group method with arithmetic
average. Cluster 1 was the most diverse with a dissimilarity range of 0.517–1.000. The level of
genetic diversity revealed in this study indicates that the studied AYB germplasm can be
exploited for genetic improvement. Additionally, the transferable markers will aid AYB
genome research and also make possible the comparative mapping between AYB and cowpea.African yam bean, AYB (Sphenostylis stenocarpa Hochst. ex. A. Rich Harms), is a tuberous
legume of tropical Africa. AYB has the potential to significantly boost food security due to
its considerable nutritional qualities. However, the crop is underutilized. To efficiently utilize
AYB genetic resources for its improvement, it is necessary to understand the crop’s diversity.
This study investigated the amplification ability of 36 cowpea simple sequence repeat (SSR)
primers across AYB genomic DNA, extracted from 67 accessions. Thirteen (36%) of the
cowpea SSRs showed transferability in AYB. Eight of these SSRs amplified above 60% of
AYB accessions and generated 55 polymorphic fragments with an average of 6.9 per primer.
Polymorphic information content ranged from 0.6691 to 0.8857 with an average of 0.7791.
This study also assessed the genetic diversity within 67 AYB accessions using eight cowpea
(Vigna unguiculata L. Walp)-derived SSR primers. The result revealed a high level of genetic
diversity with simple matching coefficient ranging from 0.458 to 1.000. A dendrogram depicting
three main clusters was generated based on unweighted pair group method with arithmetic
average. Cluster 1 was the most diverse with a dissimilarity range of 0.517–1.000. The level of
genetic diversity revealed in this study indicates that the studied AYB germplasm can be
exploited for genetic improvement. Additionally, the transferable markers will aid AYB
genome research and also make possible the comparative mapping between AYB and cowpea. |
| dc.language.iso | en |
| dc.subject | African Yam Bean |
| dc.subject | Cowpeas |
| dc.subject | Genetic Variation |
| dc.title | Analysis of genetic diversity of African yam bean using SSR markers derived from cowpea |
| dc.type | Journal Article |
| dc.description.version | Peer Review |
| cg.contributor.crp | Roots, Tubers and Bananas |
| cg.contributor.affiliation | International Institute of Tropical Agriculture |
| cg.contributor.affiliation | University of Ibadan |
| cg.contributor.affiliation | Ondo State University of Science and Technology |
| cg.coverage.region | Africa |
| cg.coverage.region | West Africa |
| cg.coverage.country | Nigeria |
| cg.isijournal | ISI Journal |
| cg.authorship.types | CGIAR and developing country institute |
| cg.iitasubject | Yam |
| cg.journal | Plant Genetic Resources |
| cg.howpublished | Formally Published |
| cg.accessibilitystatus | Limited Access |
| local.dspaceid | 76414 |
| cg.identifier.doi | https://dx.doi.org/10.1017/S1479262115000064 |
