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    Phylogeny and nitrogen fixation potential of Bradyrhizobium species isolated from the legume cover crop Pueraria phaseoloides (Roxb.) Benth. in Eastern Cameroon

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    S16ArtSarrPhylogenyInthomDev.pdf (1.253Mb)
    Date
    2015-09-25
    Author
    Sarr, S.
    Araki, S.
    Begoude, A.
    Yamakawa, T.
    Manga, G.A.
    Yamakawa, T.
    Htwe, A.Z.
    Type
    Journal Article
    Target Audience
    Scientists
    Metadata
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    Abstract/Description
    Pueraria is an herbaceous, perennial legume crop originating in Asia. Pueraria phaseoloides (Roxb.) Benth. (tropical kudzu) is frequently introduced into production systems and is used as green manure, a cover crop and a forage plant, making it important economically. We used P. phaseoloides as a trap crop to study and characterize soil rhizobia in Eastern Cameroon. Bacteria were isolated from fresh nodules collected from field-grown P. phaseoloides roots. The 16S-23S rRNA internal transcribed spacer (ITS) sequences from 30 bacterial isolates were amplified by polymerase chain reaction (PCR) and the reaction products were sequenced. Phylogenetic analysis revealed that all isolates were ascribed to the genus Bradyrhizobium and were grouped into three clusters of Bradyrhizobium sp. strains, one cluster of B. yuanmingense strains, and one cluster of B. elkanii strains. Acetylene reduction assay (ARA) results indicated that the B. yuanmingense strains had significantly higher nitrogen fixation potential and that they could be used as inoculants to enhance nitrogen fixation in Pueraria grown in Eastern Cameroon.
    https://dx.doi.org/10.1080/00380768.2015.1086279
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/945
    Digital Object Identifier (DOI)
    https://dx.doi.org/10.1080/00380768.2015.1086279
    IITA Subjects
    Grain Legumes
    Agrovoc Terms
    Pueraria Phaseoloides; Phylogeny; Bradyrhizobium
    Regions
    Africa; Central Africa
    Countries
    Cameroon
    Journals
    Soil Science and Plant Nutrition
    Collections
    • Journal and Journal Articles4835
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