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    Development of reference transcriptomes for the major field insect pests of cowpea: a toolbox for insect pest management approaches in West Africa

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    U13ArtAgunbiadeDevelopmentInthomDev.pdf (2.271Mb)
    Date
    2013-11
    Author
    Agunbiade, T.A.
    Sun, W.
    Coates, B.S.
    Djouaka, R.F.
    Tamo, M.
    Ba, Malick N.
    Binso-Dabire, L.
    Baoua, I.
    Olds, B.P.
    Pittendrigh, Barry R.
    Type
    Journal Article
    Metadata
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    Abstract/Description
    Cowpea is a widely cultivated and major nutritional source of protein for many people that live in West Africa. Annual yields and longevity of grain storage is greatly reduced by feeding damage caused by a complex of insect pests that include the pod sucking bugs, Anoplocnemis curvipes Fabricius (Hemiptera: Coreidae) and Clavigralla tomentosicollis Stål (Hemiptera: Coreidae); as well as phloem-feeding cowpea aphids, Aphis craccivora Koch (Hemiptera: Aphididae) and flower thrips, Megalurothrips sjostedti Trybom (Thysanoptera: Thripidae). Efforts to control these pests remain a challenge and there is a need to understand the structure and movement of these pest populations in order to facilitate the development of integrated pest management strategies (IPM). Molecular tools have the potential to help facilitate a better understanding of pest populations. Towards this goal, we used 454 pyrosequencing technology to generate 319,126, 176,262, 320,722 and 227,882 raw reads from A. curvipes, A. craccivora, C. tomentosicollis and M. sjostedti, respectively. The reads were de novo assembled into 11,687, 7,647,10,652 and 7,348 transcripts for A. curvipes, A. craccivora, C. tomentosicollis and M. sjostedti, respectively.Functional annotation of the resulting transcripts identified genes putatively involved in insecticide resistance, pathogen defense and immunity. Additionally, sequences that matched the primary aphid endosymbiont, Buchneraaphidicola, were identified among A. craccivora transcripts. Furthermore, 742, 97, 607 and 180 single nucleotide polymorphisms (SNPs) were respectively predicted among A. curvipes, A. craccivora, C. tomentosicollis and M.sjostedti transcripts, and will likely be valuable tools for future molecular genetic marker development. These results demonstrate that Roche 454-based transcriptome sequencing could be useful for the development of genomic resources for cowpea pest insects in West Africa.
    https://dx.doi.org/10.1371/journal.pone.0079929
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/1292
    Digital Object Identifier (DOI)
    https://dx.doi.org/10.1371/journal.pone.0079929
    IITA Subjects
    Cowpea; Grain Legumes
    Agrovoc Terms
    Pest Management; Cowpeas; Pest Control; Grain Legumes
    Regions
    Africa; West Africa
    Countries
    Benin
    Journals
    PLOS ONE
    Collections
    • Journal and Journal Articles4835
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