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Development of reference transcriptomes for the major field insect pests of cowpea: a toolbox for insect pest management approaches in West Africa

dc.contributor.authorAgunbiade, T.A.
dc.contributor.authorSun, W.
dc.contributor.authorCoates, B.S.
dc.contributor.authorDjouaka, R.F.
dc.contributor.authorTamo, M.
dc.contributor.authorBa, Malick N.
dc.contributor.authorBinso-Dabire, L.
dc.contributor.authorBaoua, I.
dc.contributor.authorOlds, B.P.
dc.contributor.authorPittendrigh, Barry R.
dc.date.accessioned2019-12-04T11:03:57Z
dc.date.available2019-12-04T11:03:57Z
dc.date.issued2013-11
dc.identifier.citationAgunbiade, T.A., Sun, W., Coates, B.S., Djouaka, R.F., Tamo, M., Ba, M.N., ... & Pittendrigh, B. R. (2013). Development of reference transcriptomes for the major field insect pests of cowpea: a toolbox for insect pest management approaches in West Africa. PLoS ONE, 8(11), 1-14.
dc.identifier.issn1932-6203
dc.identifier.urihttps://hdl.handle.net/20.500.12478/1292
dc.description.abstractCowpea is a widely cultivated and major nutritional source of protein for many people that live in West Africa. Annual yields and longevity of grain storage is greatly reduced by feeding damage caused by a complex of insect pests that include the pod sucking bugs, Anoplocnemis curvipes Fabricius (Hemiptera: Coreidae) and Clavigralla tomentosicollis Stål (Hemiptera: Coreidae); as well as phloem-feeding cowpea aphids, Aphis craccivora Koch (Hemiptera: Aphididae) and flower thrips, Megalurothrips sjostedti Trybom (Thysanoptera: Thripidae). Efforts to control these pests remain a challenge and there is a need to understand the structure and movement of these pest populations in order to facilitate the development of integrated pest management strategies (IPM). Molecular tools have the potential to help facilitate a better understanding of pest populations. Towards this goal, we used 454 pyrosequencing technology to generate 319,126, 176,262, 320,722 and 227,882 raw reads from A. curvipes, A. craccivora, C. tomentosicollis and M. sjostedti, respectively. The reads were de novo assembled into 11,687, 7,647,10,652 and 7,348 transcripts for A. curvipes, A. craccivora, C. tomentosicollis and M. sjostedti, respectively.Functional annotation of the resulting transcripts identified genes putatively involved in insecticide resistance, pathogen defense and immunity. Additionally, sequences that matched the primary aphid endosymbiont, Buchneraaphidicola, were identified among A. craccivora transcripts. Furthermore, 742, 97, 607 and 180 single nucleotide polymorphisms (SNPs) were respectively predicted among A. curvipes, A. craccivora, C. tomentosicollis and M.sjostedti transcripts, and will likely be valuable tools for future molecular genetic marker development. These results demonstrate that Roche 454-based transcriptome sequencing could be useful for the development of genomic resources for cowpea pest insects in West Africa.
dc.format.extent001-014
dc.language.isoen
dc.subjectPest Management
dc.subjectCowpeas
dc.subjectPest Control
dc.subjectGrain Legumes
dc.titleDevelopment of reference transcriptomes for the major field insect pests of cowpea: a toolbox for insect pest management approaches in West Africa
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.crpGrain Legumes
cg.contributor.affiliationUniversity of Illinois
cg.contributor.affiliationUnited States Department of Agriculture
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationInstitut de l'Environnement et de Recherches Agricoles, Burkina Faso
cg.contributor.affiliationInstitut National de la Recherche Agronomique du Niger
cg.coverage.regionAfrica
cg.coverage.regionWest Africa
cg.coverage.countryBenin
cg.isijournalISI Journal
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectCowpea
cg.iitasubjectGrain Legumes
cg.journalPLOS ONE
cg.howpublishedFormally Published
cg.accessibilitystatusOpen Access
local.dspaceid78695
cg.identifier.doihttps://dx.doi.org/10.1371/journal.pone.0079929


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