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    Unlocking the potential of tropical root crop biotechnology in east Africa by establishing a genetic transformation platform for local farmerpreferred cassava cultivars

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    Date
    2013-12
    Author
    Nyaboga, E.
    Njiru, J.
    Nguu, E.
    Gruissem, W.
    Vanderschuren, H.
    Tripathi, L.
    Type
    Journal Article
    Metadata
    Show full item record
    Abstract/Description
    CassavagenetictransformationcapacityisstillmostlyrestrictedtoadvancedlaboratoriesintheUSA,EuropeandChina;anditsimplementationandmaintenanceinAfricanlaboratorieshasremainedscarce.TheimpactoftransgenictechnologiesforgeneticimprovementofcassavawilldependlargelyonthetransferofsuchcapabilitiestoresearchersinAfrica,wherecassavahasanimportantsocioeconomicniche.Amajorconstrainttothedevelopmentofgenetictransformationtechnologiesforcassavaimprovementhasbeenthelackofanefficientandrobusttransformationandregenerationsystem.Despitethesuccessachievedingeneticmodificationoffewcassavacultivars,includingthemodelcultivar60444,transgeniccassavaproductionremainsdifficultforfarmer-preferredcultivars.Inthisstudy,aprotocolforcultivar60444developedatETHZurichwassuccessfullyimplementedandoptimizedtoestablishtransformationoffarmer-preferredcassavacultivarspopularineastAfrica.Theconditionsforproductionandproliferationoffriableembryogeniccalli(FEC)andAgrobacterium-mediatedtransformationwereoptimizedforthreeeastAfricanfarmer-preferredcultivars(Ebwanatereka,KibandamenoandSerere).Ourresultsdemonstratedtransformationefficienciesofabout14–22independenttransgeniclinesper100mgofFECforfarmer-preferredcultivarsincomparisonto28linesper100mgofthemodelcultivar60444.Thepresence,integrationandexpressionofthetransgeneswereconfirmedbyPCR,SouthernblotanalysisandhistochemicalGUSassay.ThisstudyreportstheestablishmentofacassavatransformationplatformatInternationalInstituteofTropicalAgriculture(IITA)hostedbyBioscienceseasternandcentralAfrica(BecA)hubinKenyaandprovidesthebasisfortransferringimportanttraitssuchasvirusresistanceandprolongedshelf-lifetofarmer-preferredcultivarsineastAfrica.Weanticipatethatsuchplatformwillalsobeinstrumentaltotransfertechnologiestonationalagriculturalresearchsystems(NARS)insub-SaharanAfrica.
    https://dx.doi.org/10.3389/fpls.2013.00526
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/1305
    Digital Object Identifier (DOI)
    https://dx.doi.org/10.3389/fpls.2013.00526
    IITA Subjects
    Cassava
    Agrovoc Terms
    Cassava; Genetic Transformation; Agrobacterium Tumefaciens; Cultivars
    Regions
    Africa; East Africa
    Countries
    Kenya
    Journals
    Frontiers in Plant Science
    Collections
    • Journal and Journal Articles4835
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