| dc.contributor.author | Nyaboga, E. |
| dc.contributor.author | Njiru, J. |
| dc.contributor.author | Nguu, E. |
| dc.contributor.author | Gruissem, W. |
| dc.contributor.author | Vanderschuren, H. |
| dc.contributor.author | Tripathi, L. |
| dc.date.accessioned | 2019-12-04T11:03:59Z |
| dc.date.available | 2019-12-04T11:03:59Z |
| dc.date.issued | 2013-12 |
| dc.identifier.citation | Nyaboga, E., Njiru, J., Nguu, E., Gruissem, W., Vanderschuren, H. & Tripathi, L. (2013). Unlocking the potential of tropical root crop biotechnology in east Africa by establishing a genetic transformation platform for local farmer-preferred cassava cultivars. Frontiers in Plant Science, 4(526), 1-11. |
| dc.identifier.issn | 1664-462X |
| dc.identifier.uri | https://hdl.handle.net/20.500.12478/1305 |
| dc.description.abstract | CassavagenetictransformationcapacityisstillmostlyrestrictedtoadvancedlaboratoriesintheUSA,EuropeandChina;anditsimplementationandmaintenanceinAfricanlaboratorieshasremainedscarce.TheimpactoftransgenictechnologiesforgeneticimprovementofcassavawilldependlargelyonthetransferofsuchcapabilitiestoresearchersinAfrica,wherecassavahasanimportantsocioeconomicniche.Amajorconstrainttothedevelopmentofgenetictransformationtechnologiesforcassavaimprovementhasbeenthelackofanefficientandrobusttransformationandregenerationsystem.Despitethesuccessachievedingeneticmodificationoffewcassavacultivars,includingthemodelcultivar60444,transgeniccassavaproductionremainsdifficultforfarmer-preferredcultivars.Inthisstudy,aprotocolforcultivar60444developedatETHZurichwassuccessfullyimplementedandoptimizedtoestablishtransformationoffarmer-preferredcassavacultivarspopularineastAfrica.Theconditionsforproductionandproliferationoffriableembryogeniccalli(FEC)andAgrobacterium-mediatedtransformationwereoptimizedforthreeeastAfricanfarmer-preferredcultivars(Ebwanatereka,KibandamenoandSerere).Ourresultsdemonstratedtransformationefficienciesofabout14–22independenttransgeniclinesper100mgofFECforfarmer-preferredcultivarsincomparisonto28linesper100mgofthemodelcultivar60444.Thepresence,integrationandexpressionofthetransgeneswereconfirmedbyPCR,SouthernblotanalysisandhistochemicalGUSassay.ThisstudyreportstheestablishmentofacassavatransformationplatformatInternationalInstituteofTropicalAgriculture(IITA)hostedbyBioscienceseasternandcentralAfrica(BecA)hubinKenyaandprovidesthebasisfortransferringimportanttraitssuchasvirusresistanceandprolongedshelf-lifetofarmer-preferredcultivarsineastAfrica.Weanticipatethatsuchplatformwillalsobeinstrumentaltotransfertechnologiestonationalagriculturalresearchsystems(NARS)insub-SaharanAfrica. |
| dc.format.extent | 11 |
| dc.language.iso | en |
| dc.subject | Cassava |
| dc.subject | Genetic Transformation |
| dc.subject | Agrobacterium Tumefaciens |
| dc.subject | Cultivars |
| dc.title | Unlocking the potential of tropical root crop biotechnology in east Africa by establishing a genetic transformation platform for local farmerpreferred cassava cultivars |
| dc.type | Journal Article |
| dc.description.version | Peer Review |
| cg.contributor.crp | Roots, Tubers and Bananas |
| cg.contributor.affiliation | International Institute of Tropical Agriculture |
| cg.contributor.affiliation | Eidgenössische Technische Hochschule Zürich |
| cg.contributor.affiliation | University of Nairobi |
| cg.coverage.region | Africa |
| cg.coverage.region | East Africa |
| cg.coverage.country | Kenya |
| cg.isijournal | ISI Journal |
| cg.authorship.types | CGIAR and developing country institute |
| cg.iitasubject | Cassava |
| cg.journal | Frontiers in Plant Science |
| cg.howpublished | Formally Published |
| cg.accessibilitystatus | Limited Access |
| local.dspaceid | 78708 |
| cg.identifier.doi | https://dx.doi.org/10.3389/fpls.2013.00526 |
