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    Survey, molecular detection and characterization of geminiviruses associated with cassava mosaic disease in Zambia

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    S16ArtMulengaSurveyInthomDev.pdf (629.3Kb)
    Date
    2016-07
    Author
    Mulenga, R.M.
    Legg, J.P.
    Ndunguru, J.
    Miano, D.W.
    Mutitu, E.W.
    Chikoti, Patrick Chiza
    Alabi, O.J.
    Type
    Journal Article
    Target Audience
    Scientists
    Metadata
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    Abstract/Description
    A survey was conducted from April to May 2014 in 214 farmers’ fields located across six major cassava-producing provinces (Western, Northwestern, Northern, Luapula, Lusaka, and Eastern) of Zambia to determine the status of cassava mosaic disease (CMD) and the species diversity of associated cassava mosaic geminiviruses (CMG). Mean CMD incidence varied across all six provinces but was greatest in Lusaka Province (81%) and least in Northern Province (44%). Mean CMD severity varied slightly between provinces, ranging from 2.78 in Eastern Province to 3.00 in Northwestern Province. Polymerase chain reaction discrimination of 226 survey samples, coupled with complete DNA-A genome sequence analysis, revealed the presence of African cassava mosaic virus (ACMV), East African cassava mosaic virus (EACMV), and East African cassava mosaic Malawi virus (EACMMV) as single or mixed infections of different proportions. Single-virus infections were predominant, occurring in 62.8% (ACMV), 5.8% (EACMMV), and 2.2% (EACMV) of samples relative to mixed-virus infections, which occurred in 19.5% (ACMV + EACMMV), 0.4% (ACMV + EACMV), and 0.9% (ACMV + EACMV + EACMMV) of samples. Phylogenetic analysis revealed the segregation of virus isolates from Zambia into clades specific to ACMV, EACMV, and EACMMV, further confirming the presence of all three viruses in Zambia. The results point to a greater diversity of CMG across major cassava-growing provinces of Zambia and implicate contaminated cassava cuttings in disease spread.
    https://dx.doi.org/10.1094/PDIS-10-15-1170-RE
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/1325
    Digital Object Identifier (DOI)
    https://dx.doi.org/10.1094/PDIS-10-15-1170-RE
    IITA Subjects
    Cassava; Plant Diseases
    Agrovoc Terms
    Geminiviruses; Epidemiology; Genetic Variation; Cassava
    Regions
    Africa; Southern Africa
    Countries
    Zambia
    Journals
    Plant Disease
    Collections
    • Journal and Journal Articles4835
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