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Survey, molecular detection and characterization of geminiviruses associated with cassava mosaic disease in Zambia

dc.contributor.authorMulenga, R.M.
dc.contributor.authorLegg, J.P.
dc.contributor.authorNdunguru, J.
dc.contributor.authorMiano, D.W.
dc.contributor.authorMutitu, E.W.
dc.contributor.authorChikoti, Patrick Chiza
dc.contributor.authorAlabi, O.J.
dc.date.accessioned2019-12-04T11:04:03Z
dc.date.available2019-12-04T11:04:03Z
dc.date.issued2016-07
dc.identifier.citationMulenga, R.M., Legg, J., Ndunguru, J., Miano, D.W., Mutitu, E.W., Chikoti, Patrick Chiza & Alabi, O.J. (2016). Survey, molecular detection and characterization of geminiviruses associated with cassava mosaic disease in Zambia. Plant Disease, 100(7), 1379-1387.
dc.identifier.issn0191-2917
dc.identifier.urihttps://hdl.handle.net/20.500.12478/1325
dc.description.abstractA survey was conducted from April to May 2014 in 214 farmers’ fields located across six major cassava-producing provinces (Western, Northwestern, Northern, Luapula, Lusaka, and Eastern) of Zambia to determine the status of cassava mosaic disease (CMD) and the species diversity of associated cassava mosaic geminiviruses (CMG). Mean CMD incidence varied across all six provinces but was greatest in Lusaka Province (81%) and least in Northern Province (44%). Mean CMD severity varied slightly between provinces, ranging from 2.78 in Eastern Province to 3.00 in Northwestern Province. Polymerase chain reaction discrimination of 226 survey samples, coupled with complete DNA-A genome sequence analysis, revealed the presence of African cassava mosaic virus (ACMV), East African cassava mosaic virus (EACMV), and East African cassava mosaic Malawi virus (EACMMV) as single or mixed infections of different proportions. Single-virus infections were predominant, occurring in 62.8% (ACMV), 5.8% (EACMMV), and 2.2% (EACMV) of samples relative to mixed-virus infections, which occurred in 19.5% (ACMV + EACMMV), 0.4% (ACMV + EACMV), and 0.9% (ACMV + EACMV + EACMMV) of samples. Phylogenetic analysis revealed the segregation of virus isolates from Zambia into clades specific to ACMV, EACMV, and EACMMV, further confirming the presence of all three viruses in Zambia. The results point to a greater diversity of CMG across major cassava-growing provinces of Zambia and implicate contaminated cassava cuttings in disease spread.
dc.format.extent1379-1387
dc.language.isoen
dc.subjectGeminiviruses
dc.subjectEpidemiology
dc.subjectGenetic Variation
dc.subjectCassava
dc.titleSurvey, molecular detection and characterization of geminiviruses associated with cassava mosaic disease in Zambia
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.crpRoots, Tubers and Bananas
cg.contributor.affiliationZambia Agricultural Research Institute
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationMikocheni Agricultural Research Institute, Tanzania
cg.contributor.affiliationUniversity of Nairobi
cg.contributor.affiliationTexas A&M University
cg.coverage.regionAfrica
cg.coverage.regionSouthern Africa
cg.coverage.countryZambia
cg.isijournalISI Journal
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectCassava
cg.iitasubjectPlant Diseases
cg.journalPlant Disease
cg.howpublishedFormally Published
cg.accessibilitystatusLimited Access
local.dspaceid79147
cg.targetaudienceScientists
cg.identifier.doihttps://dx.doi.org/10.1094/PDIS-10-15-1170-RE


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