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Diversity in secondary metabolites including mycotoxins from strains of aspergillus section nigri isolated from raw cashew nuts from Benin, West Africa

dc.contributor.authorLamboni, Y.
dc.contributor.authorNielsen, K.F.
dc.contributor.authorLinnemann, A.
dc.contributor.authorGezgin, Y.
dc.contributor.authorHell, K.
dc.contributor.authorNout, R.M.
dc.contributor.authorSmid, E.J.
dc.contributor.authorTamo, M.
dc.contributor.authorBoekel, M.A. van
dc.contributor.authorHoof, J.B.
dc.contributor.authorFrisvad, J.C.
dc.date.accessioned2019-12-04T11:04:41Z
dc.date.available2019-12-04T11:04:41Z
dc.date.issued2016-10-21
dc.identifier.citationLamboni, Y., Nielsen, K.F., Linnemann, A., Gezgin, Y., Hell, K., Nout, R.M., ... & Frisvad, J.C. (2016). Diversity in secondary metabolites including mycotoxins from strains of aspergillus section nigri isolated from raw cashew nuts from Benin, West Africa. PLoS One, 11(10), 1-14.
dc.identifier.issn1932-6203
dc.identifier.urihttps://hdl.handle.net/20.500.12478/1483
dc.descriptionOpen access Journal
dc.description.abstractIn a previous study, raw cashew kernels were assayed for the fungal contamination focusing on strains belonging to the genus Aspergillus and on aflatoxins producers. These samples showed high contamination with Aspergillus section Nigri species and absence of aflatoxins. To investigate the diversity of secondary metabolites, including mycotoxins, the species of A. section Nigri may produce and thus threaten to contaminate the raw cashew kernels, 150 strains were isolated from cashew samples and assayed for their production of secondary metabolites using liquid chromatography high resolution mass spectrometry (LC-HRMS). Seven species of black Aspergilli were isolated based on morphological and chemical identification: A. tubingensis (44%), A. niger (32%), A. brasiliensis (10%), A. carbonarius (8.7%), A. luchuensis (2.7%), A. aculeatus (2%) and A. aculeatinus (0.7%). From these, 45 metabolites and their isomers were identified. Aurasperone and pyranonigrin A, produced by all species excluding A. aculeatus and A. aculeatinus, were most prevalent and were encountered in 146 (97.3%) and 145 (95.7%) isolates, respectively. Three mycotoxins groups were detected: fumonisins (B2 and B4) (2.7%) ochratoxin A (13.3%), and secalonic acids (2%), indicating that these mycotoxins could occur in raw cashew nuts. Thirty strains of black Aspergilli were randomly sampled for verification of species identity based on sequences of β-tubulin and calmodulin genes. Among them, 27 isolates were positive to the primers used and 11 were identified as A. niger, 7 as A. tubingensis, 6 as A. carbonarius, 2 as A. luchuensis and 1 as A. welwitschiae confirming the species names as based on morphology and chemical features. These strains clustered in 5 clades in A. section Nigri. Chemical profile clustering also showed also 5 groups confirming the species specific metabolites production.
dc.format.extent1-14
dc.language.isoen
dc.subjectMycotoxins
dc.subjectAspergillus
dc.subjectMetabolites
dc.subjectNuts
dc.subjectCashew
dc.titleDiversity in secondary metabolites including mycotoxins from strains of aspergillus section nigri isolated from raw cashew nuts from Benin, West Africa
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationTechnical University of Denmark
cg.contributor.affiliationWageningen University and Research Centre
cg.contributor.affiliationEge University
cg.coverage.regionAfrica
cg.coverage.regionWest Africa
cg.coverage.countryBenin
cg.isijournalISI Journal
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectFood Security
cg.iitasubjectPests Of Plants
cg.journalPloS ONE
cg.howpublishedFormally Published
cg.accessibilitystatusOpen Access
local.dspaceid80668
cg.targetaudienceScientists
cg.identifier.doihttps://dx.doi.org/10.1371/journal.pone.0164310


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