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Cassava haplotype map highlights fixation of deleterious mutations during clonal propagation

dc.contributor.authorRamu, P.
dc.contributor.authorEsuma, W.
dc.contributor.authorKawuki, R.
dc.contributor.authorRabbi, Ismail Y
dc.contributor.authorEgesi, Chiedozie N.
dc.contributor.authorBredeson, J.V.
dc.contributor.authorBart, R.S.
dc.contributor.authorVerma, J.
dc.contributor.authorBuckler, E.S.
dc.contributor.authorLu, F
dc.date.accessioned2019-12-04T11:08:14Z
dc.date.available2019-12-04T11:08:14Z
dc.date.issued2017-04-17
dc.identifier.citationRamu, P., Esuma, W., Kawuki, R., Rabbi, I.Y., Egesi, C., Bredeson, J.V., ... & Lu, F. (2017). Cassava haplotype map highlights fixation of deleterious mutations during clonal propagation. Nature Genetics.
dc.identifier.issn1061-4036
dc.identifier.urihttps://hdl.handle.net/20.500.12478/1767
dc.descriptionArticle purchased; Published online: 17 April 2017
dc.description.abstractCassava (Manihot esculenta Crantz) is an important staple food crop in Africa and South America; however, ubiquitous deleterious mutations may severely decrease its fitness. To evaluate these deleterious mutations, we constructed a cassava haplotype map through deep sequencing 241 diverse accessions and identified >28 million segregating variants. We found that (i) although domestication has modified starch and ketone metabolism pathways to allow for human consumption, the concomitant bottleneck and clonal propagation have resulted in a large proportion of fixed deleterious amino acid changes, increased the number of deleterious alleles by 26%, and shifted the mutational burden toward common variants; (ii) deleterious mutations have been ineffectively purged, owing to limited recombination in the cassava genome; (iii) recent breeding efforts have maintained yield by masking the most damaging recessive mutations in the heterozygous state but have been unable to purge the mutation burden; such purging should be a key target in future cassava breeding.
dc.description.sponsorshipBill & Melinda Gates Foundation
dc.format.extent1-7
dc.language.isoen
dc.subjectGenomics
dc.subjectPlant Genetics
dc.subjectPopulation Genetics
dc.subjectCassava
dc.subjectDeleterious Mutations
dc.subjectHaplotype Map
dc.subjectClonal Propagation
dc.titleCassava haplotype map highlights fixation of deleterious mutations during clonal propagation
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.crpRoots, Tubers and Bananas
cg.contributor.affiliationCornell University
cg.contributor.affiliationNational Crops Resources Research Institute, Uganda
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationNational Root Crops Research Institute, Nigeria
cg.contributor.affiliationUniversity of California
cg.contributor.affiliationDonald Danforth Plant Science Center
cg.contributor.affiliationUnited States Department of Agriculture
cg.researchthemeBIOTECH & PLANT BREEDING
cg.isijournalISI Journal
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectCassava
cg.iitasubjectPlant Breeding
cg.journalNature Genetics
cg.howpublishedFormally Published
cg.accessibilitystatusOpen Access
local.dspaceid83176
cg.targetaudienceScientists
cg.identifier.doihttp://dx.doi.org/10.1038/ng.3845


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