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    Morphological and molecular characterisation of Scutellonema species from yam (Dioscorea spp.) and a key to the species of the genus

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    U17ArtKolombiaMorphologicalInthomNodev.pdf (5.661Mb)
    Date
    2017
    Author
    Kolombia, Y.A.
    Karssen, G.
    Viaene, N.
    Kumar, P.L.
    Joos, L.
    Coyne, D.L.
    Bert, W.
    Type
    Journal Article
    Target Audience
    Scientists
    Metadata
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    Abstract/Description
    The yam nematode, Scutellonema bradys, is a major threat to yam (Dioscorea spp.) production across yam-growing regions. In West Africa, this species cohabits with many morphologically similar congeners and, consequently, its accurate diagnosis is essential for control and for monitoring its movement. In the present study, 46 Scutellonema populations collected from yam rhizosphere and yam tubers in different agro-ecological zones in Ghana and Nigeria were characterised by their morphological features and by sequencing of the D2-D3 region of the 28S rDNA gene and the mitochondrial COI genes. Molecular phylogeny, molecular species delimitation and morphology revealed S. bradys, S. cavenessi, S. clathricaudatum and three undescribed species from yam rhizosphere. Only S. bradys was identified from yam tuber tissue, however. For barcoding and identifying Scutellonema spp., the most suitable marker used was the COI gene. Additionally, 99 new Scutellonema sequences were generated using populations obtained also from banana, carrot, maize and tomato, including the first for S. paralabiatum and S. clathricaudatum, enabling the development of a dichotomous key for identification of Scutellonema spp. The implications of these results are discussed.
    https://dx.doi.org/10.1163/15685411-00003084
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/2004
    Digital Object Identifier (DOI)
    https://dx.doi.org/10.1163/15685411-00003084
    IITA Subjects
    Yam
    Agrovoc Terms
    Scutellonema Bradys; Phylogeny; Coi; Diagnostics; Identification; Key; Cavenessi; Scutellonema Clathricaudatum; Morphological Traits
    Regions
    Africa; West Africa
    Countries
    Ghana; Nigeria
    Journals
    Nematology
    Collections
    • Journal and Journal Articles5283
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