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Morphological and molecular characterisation of Scutellonema species from yam (Dioscorea spp.) and a key to the species of the genus

dc.contributor.authorKolombia, Y.A.
dc.contributor.authorKarssen, G.
dc.contributor.authorViaene, N.
dc.contributor.authorKumar, P.L.
dc.contributor.authorJoos, L.
dc.contributor.authorCoyne, D.L.
dc.contributor.authorBert, W.
dc.date.accessioned2019-12-04T11:09:14Z
dc.date.available2019-12-04T11:09:14Z
dc.date.issued2017
dc.identifier.citationKolombia, Y.A., Karssen, G., Viaene, N., Kumar, P.L., Joos, L., Coyne, D.L. & Bert, W. (2017). Morphological and molecular characterisation of Scutellonema species from yam (Dioscorea spp.) and a key to the species of the genus. Nematology, 1-37.
dc.identifier.issn1388-5545
dc.identifier.urihttps://hdl.handle.net/20.500.12478/2004
dc.descriptionPublished online: July 2017
dc.description.abstractThe yam nematode, Scutellonema bradys, is a major threat to yam (Dioscorea spp.) production across yam-growing regions. In West Africa, this species cohabits with many morphologically similar congeners and, consequently, its accurate diagnosis is essential for control and for monitoring its movement. In the present study, 46 Scutellonema populations collected from yam rhizosphere and yam tubers in different agro-ecological zones in Ghana and Nigeria were characterised by their morphological features and by sequencing of the D2-D3 region of the 28S rDNA gene and the mitochondrial COI genes. Molecular phylogeny, molecular species delimitation and morphology revealed S. bradys, S. cavenessi, S. clathricaudatum and three undescribed species from yam rhizosphere. Only S. bradys was identified from yam tuber tissue, however. For barcoding and identifying Scutellonema spp., the most suitable marker used was the COI gene. Additionally, 99 new Scutellonema sequences were generated using populations obtained also from banana, carrot, maize and tomato, including the first for S. paralabiatum and S. clathricaudatum, enabling the development of a dichotomous key for identification of Scutellonema spp. The implications of these results are discussed.
dc.description.sponsorshipBill & Melinda Gates Foundation
dc.format.extent1-37
dc.language.isoen
dc.subjectScutellonema Bradys
dc.subjectPhylogeny
dc.subjectCoi
dc.subjectDiagnostics
dc.subjectIdentification
dc.subjectKey
dc.subjectCavenessi
dc.subjectScutellonema Clathricaudatum
dc.subjectMorphological Traits
dc.titleMorphological and molecular characterisation of Scutellonema species from yam (Dioscorea spp.) and a key to the species of the genus
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.crpRoots, Tubers and Bananas
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationNational Plant Protection Organization, Netherlands
cg.contributor.affiliationFlanders Research Institute for Agriculture, Fisheries and Food, Belgium
cg.coverage.regionAfrica
cg.coverage.regionWest Africa
cg.coverage.countryGhana
cg.coverage.countryNigeria
cg.isijournalISI Journal
cg.authorship.typesCGIAR and advanced research institute
cg.iitasubjectYam
cg.journalNematology
cg.howpublishedFormally Published
cg.accessibilitystatusOpen Access
local.dspaceid85681
cg.targetaudienceScientists
cg.identifier.doihttps://dx.doi.org/10.1163/15685411-00003084


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