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    Diversity of root-knot nematodes associated with tubers of yam (Dioscorea spp.) established using isozyme analysis and mitochondrial DNA-based identification

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    S17ArtKolombiaDiversityInthomNodev.pdf (1.857Mb)
    Date
    2017-06
    Author
    Kolombia, Y.A.
    Karssen, G.
    Viaene, N.
    Kumar, P.L.
    Sutter, N. de
    Joos, L.
    Coyne, D.L.
    Bert, W.
    Type
    Journal Article
    Target Audience
    Scientists
    Metadata
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    Abstract/Description
    The root-knot nematodes (RKN), Meloidogyne spp., represent an important threat to yam (Dioscorea spp.) production in West Africa. With the aim to establish the diversity of RKN species affecting yam tubers, for control and resistance screening purposes, surveys were conducted in the main yam producing areas of Nigeria. Galled tubers (N = 48) were collected from farmers’ stores and markets in nine states in Nigeria and in one district in Ghana. RKN isolated from yam tubers were identified using enzyme phenotyping (esterase and malate dehydrogenase) and mitochondrial DNA (mtDNA) NADH dehydrogenase subunit 5 (Nad5) barcoding. Examination of 48 populations revealed that yam tubers were infested by Meloidogyne incognita (69%), followed by M. javanica (13%), M. enterolobii (2%), and M. arenaria (2%). Most of the tubers sampled (86%) were infected by a single species, and multiple species of RKN were detected in 14% of the samples. Results of both identification methods revealed the same species, confirming their accuracy for the identification of these tropical RKN species. In addition to M. incognita, M. javanica, and M. enterolobii, we report for the first time M. arenaria infecting yam tubers in Nigeria. This finding extends the list of yam pests and calls for caution when developing practices for yam pest management.
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/2012
    IITA Subjects
    Disease Control; Plant Diseases; Plant Genetic Resources; Yam
    Agrovoc Terms
    Esterase; Isozymes; Meloidogyne Arenaria; Meloidogyne Incognita; Meloidogyne Javanica; Malate Dehydrogenase; Meloidogyne; Yams; Dna; Root-Knot Nematodes; Nad5; Sequences
    Regions
    Africa; West Africa
    Countries
    Nigeria
    Journals
    Journal of Nematology
    Collections
    • Journal and Journal Articles4835
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