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Diversity of root-knot nematodes associated with tubers of yam (Dioscorea spp.) established using isozyme analysis and mitochondrial DNA-based identification

dc.contributor.authorKolombia, Y.A.
dc.contributor.authorKarssen, G.
dc.contributor.authorViaene, N.
dc.contributor.authorKumar, P.L.
dc.contributor.authorSutter, N. de
dc.contributor.authorJoos, L.
dc.contributor.authorCoyne, D.L.
dc.contributor.authorBert, W.
dc.date.accessioned2019-12-04T11:09:16Z
dc.date.available2019-12-04T11:09:16Z
dc.date.issued2017-06
dc.identifier.citationKolombia, Y.A., Karssen, G., Viaene, N., Kumar, P.L., de Sutter, N., Joos, L., ... & Bert, W. (2017). Diversity of root-knot nematodes associated with tubers of yam (Dioscorea spp.) established using isozyme analysis and mitochondrial DNA-based identification. Journal of Nematology, 49(2), 177-188.
dc.identifier.issn0022-300X
dc.identifier.urihttps://hdl.handle.net/20.500.12478/2012
dc.description.abstractThe root-knot nematodes (RKN), Meloidogyne spp., represent an important threat to yam (Dioscorea spp.) production in West Africa. With the aim to establish the diversity of RKN species affecting yam tubers, for control and resistance screening purposes, surveys were conducted in the main yam producing areas of Nigeria. Galled tubers (N = 48) were collected from farmers’ stores and markets in nine states in Nigeria and in one district in Ghana. RKN isolated from yam tubers were identified using enzyme phenotyping (esterase and malate dehydrogenase) and mitochondrial DNA (mtDNA) NADH dehydrogenase subunit 5 (Nad5) barcoding. Examination of 48 populations revealed that yam tubers were infested by Meloidogyne incognita (69%), followed by M. javanica (13%), M. enterolobii (2%), and M. arenaria (2%). Most of the tubers sampled (86%) were infected by a single species, and multiple species of RKN were detected in 14% of the samples. Results of both identification methods revealed the same species, confirming their accuracy for the identification of these tropical RKN species. In addition to M. incognita, M. javanica, and M. enterolobii, we report for the first time M. arenaria infecting yam tubers in Nigeria. This finding extends the list of yam pests and calls for caution when developing practices for yam pest management.
dc.description.sponsorshipBill & Melinda Gates Foundation
dc.format.extent177-188
dc.language.isoen
dc.subjectEsterase
dc.subjectIsozymes
dc.subjectMeloidogyne Arenaria
dc.subjectMeloidogyne Incognita
dc.subjectMeloidogyne Javanica
dc.subjectMalate Dehydrogenase
dc.subjectMeloidogyne
dc.subjectYams
dc.subjectDna
dc.subjectRoot-Knot Nematodes
dc.subjectNad5
dc.subjectSequences
dc.titleDiversity of root-knot nematodes associated with tubers of yam (Dioscorea spp.) established using isozyme analysis and mitochondrial DNA-based identification
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.crpRoots, Tubers and Bananas
cg.contributor.affiliationGhent University
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationNational Plant Protection Organization, Netherlands
cg.contributor.affiliationFlanders Research Institute for Agriculture, Fisheries and Food, Belgium
cg.coverage.regionAfrica
cg.coverage.regionWest Africa
cg.coverage.countryNigeria
cg.isijournalISI Journal
cg.authorship.typesCGIAR and advanced research institute
cg.iitasubjectDisease Control
cg.iitasubjectPlant Diseases
cg.iitasubjectPlant Genetic Resources
cg.iitasubjectYam
cg.journalJournal of Nematology
cg.howpublishedFormally Published
cg.accessibilitystatusLimited Access
local.dspaceid85748
cg.targetaudienceScientists


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