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    Molecular characterization of atoxigenic strains for biological control of aflatoxins in Nigeria

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    S10ArtDonnerMolecularInthomNodev.pdf (716.1Kb)
    Date
    2010
    Author
    Donner, M.
    Atehnkeng, J.
    Sikora, R.A.
    Bandyopadhyay, Ranajit
    Cotty, P.J.
    Type
    Journal Article
    Target Audience
    Scientists
    Metadata
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    Abstract/Description
    Abstract Aflatoxins are highly toxic carcinogens produced by several species in Aspergillus section Flavi. Strains of A. flavus that do not produce aflatoxins, called atoxigenic strains, have been used commercially in North America as tools for limiting aflatoxin contamination. A similar aflatoxin management strategy is being pursued in Nigeria. In the current study, loci across the 68 kb aflatoxin biosynthesis gene cluster were compared among 18 atoxigenic and two aflatoxin-producing vegetative compatibility groups (VCGs) from Nigeria and an atoxigenic VCG used commercially in North America. Five of the atoxigenic VCGs had large deletions (37–65 kb) extending from the teleomeric side of the aflatoxin biosynthesis cluster. In one VCG (AV0222) the deletion extended through the cluster to the adjacent sugar cluster. The remaining twelve atoxigenic VCGs, including the VCG used for aflatoxin management in North America, contained all the aflatoxin pathway genes, but with defects. Two observations support the long-term persistence of atoxigenicity within A. flavus: first, a comparison of pathway genes revealed more changes in atoxigenic than in aflatoxin-producing isolates relative to the aflatoxin-producing strain NRRL 3357; and second, several non-synonymous changes are unique to atoxigenics. Atoxigenic VCG diversity was assessed with phylogenetic analyses. Although some atoxigenics share relatively recent ancestry, several are more closely related to aflatoxin producers than to other atoxigenics. The current study demonstrates VCGs of A. flavus in West Africa with diverse mechanisms of atoxigenicity and potential value in aflatoxin management programmes.
    http://dx.doi.org/10.1080/19440040903551954
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/2134
    Digital Object Identifier (DOI)
    http://dx.doi.org/10.1080/19440040903551954
    IITA Subjects
    Aflatoxin; Disease Control; Food Security
    Agrovoc Terms
    Polymerase Chain Reaction; Mycology; Aflatoxins; Cereals; Health Significance; Aspergillus Flavi; Atoxigenic Strains; Vegetative Compatibility Groups
    Regions
    Africa; West Africa
    Countries
    Nigeria
    Journals
    Food Additives and Contaminants
    Collections
    • Journal and Journal Articles5283
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