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Molecular characterization of atoxigenic strains for biological control of aflatoxins in Nigeria

dc.contributor.authorDonner, M.
dc.contributor.authorAtehnkeng, J.
dc.contributor.authorSikora, R.A.
dc.contributor.authorBandyopadhyay, Ranajit
dc.contributor.authorCotty, P.J.
dc.date.accessioned2019-12-04T11:10:25Z
dc.date.available2019-12-04T11:10:25Z
dc.date.issued2010
dc.identifier.citationDonner, M., Atehnkeng, J., Sikora, R.A., Bandyopadhyay, R. & Cotty, P.J. (2010). Molecular characterization of atoxigenic strains for biological control of aflatoxins in Nigeria. Food Additives and Contaminants, 27(5), 576-590.
dc.identifier.issn1944-0049
dc.identifier.urihttps://hdl.handle.net/20.500.12478/2134
dc.descriptionPublished online: 07 May 2010
dc.description.abstractAbstract Aflatoxins are highly toxic carcinogens produced by several species in Aspergillus section Flavi. Strains of A. flavus that do not produce aflatoxins, called atoxigenic strains, have been used commercially in North America as tools for limiting aflatoxin contamination. A similar aflatoxin management strategy is being pursued in Nigeria. In the current study, loci across the 68 kb aflatoxin biosynthesis gene cluster were compared among 18 atoxigenic and two aflatoxin-producing vegetative compatibility groups (VCGs) from Nigeria and an atoxigenic VCG used commercially in North America. Five of the atoxigenic VCGs had large deletions (37–65 kb) extending from the teleomeric side of the aflatoxin biosynthesis cluster. In one VCG (AV0222) the deletion extended through the cluster to the adjacent sugar cluster. The remaining twelve atoxigenic VCGs, including the VCG used for aflatoxin management in North America, contained all the aflatoxin pathway genes, but with defects. Two observations support the long-term persistence of atoxigenicity within A. flavus: first, a comparison of pathway genes revealed more changes in atoxigenic than in aflatoxin-producing isolates relative to the aflatoxin-producing strain NRRL 3357; and second, several non-synonymous changes are unique to atoxigenics. Atoxigenic VCG diversity was assessed with phylogenetic analyses. Although some atoxigenics share relatively recent ancestry, several are more closely related to aflatoxin producers than to other atoxigenics. The current study demonstrates VCGs of A. flavus in West Africa with diverse mechanisms of atoxigenicity and potential value in aflatoxin management programmes.
dc.description.sponsorshipFederal Ministry for Economic Cooperation and Development, Germany
dc.format.extent576-590
dc.language.isoen
dc.subjectPolymerase Chain Reaction
dc.subjectMycology
dc.subjectAflatoxins
dc.subjectCereals
dc.subjectHealth Significance
dc.subjectAspergillus Flavi
dc.subjectAtoxigenic Strains
dc.subjectVegetative Compatibility Groups
dc.titleMolecular characterization of atoxigenic strains for biological control of aflatoxins in Nigeria
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.affiliationUniversity of Bonn
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationUniversity of Arizona
cg.coverage.regionAfrica
cg.coverage.regionWest Africa
cg.coverage.countryNigeria
cg.isijournalISI Journal
cg.authorship.typesCGIAR and advanced research institute
cg.iitasubjectAflatoxin
cg.iitasubjectDisease Control
cg.iitasubjectFood Security
cg.journalFood Additives and Contaminants
cg.howpublishedFormally Published
cg.accessibilitystatusLimited Access
local.dspaceid90700
cg.targetaudienceScientists
cg.identifier.doihttp://dx.doi.org/10.1080/19440040903551954


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