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    Secondary metabolite profile and phytotoxic activity of genetically distinct forms of Colletotrichum gloeosporioides from yam (Dioscorea spp.)

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    S09ArtAbangSecondaryInthomNodev.pdf (660.8Kb)
    Date
    2009
    Author
    Abang, Mathew M.
    Abraham, W.R.
    Asiedu, Robert
    Hoffmann, P.
    Wolf, G.
    Winter, S.
    Type
    Journal Article
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    Abstract/Description
    Highly virulent, slow-growing grey (SGG); moderately virulent, fast-growing salmon (FGS); and avirulent/weakly virulent, fast-growing grey (FGG) forms of Colletotrichum gloeosporioides have been described from yam (Dioscorea spp.), but little is known about their chemodiversity or the role of toxins in their pathogenesis. Secondary metabolite profiles in high performance tlc (hptlc) showed that the pathogenic SGG and FGS forms have a chemotype (A or B) that is distinct from the non-pathogenic FGG form (chemotype C). Crude extracts of 35-d-old Czapek–Dox yeast broth cultures of FGS and SGG isolates caused tissue necrosis on treated yam leaves but not those of FGG isolates. Extracts from uninoculated broth cultures showed no phytotoxic activity. Toxicity of the culture filtrate was not host specific and toxic substances were thermostable. Dioscorea genotypes with varying levels of resistance to anthracnose differed in their sensitivity to crude toxin extract of FGS (Cg33) and SGG (Cg25) isolates, indicating that these extracts may be useful in evaluating host resistance to anthracnose in vitro. Analysis of two toxin fractions unique to the pathogenic FGS and SGG forms using hlpc, mass spectrometry and nuclear magnetic resonance suggested the presence of a low molecular weight amide peptide. However, possibly due to low yield and the presence of impurities, the chemical structure of the compound(s) could not be fully elucidated.
    https://dx.doi.org/10.1016/j.mycres.2008.09.004
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/2481
    Digital Object Identifier (DOI)
    https://dx.doi.org/10.1016/j.mycres.2008.09.004
    IITA Subjects
    Yam; Plant Diseases; Tissue Culture; Genetic Improvement
    Agrovoc Terms
    Anthracnose; Chemotype; Chromatography; Colletotrichum Gloeosporioides; Dioscorea Spp.; Mass Spectrometry; Metabolite Profiling; Nmr; Phytotoxin
    Regions
    Acp; Europe
    Countries
    Germany
    Journals
    Mycological Research
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    • Journal and Journal Articles5283
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