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Secondary metabolite profile and phytotoxic activity of genetically distinct forms of Colletotrichum gloeosporioides from yam (Dioscorea spp.)

dc.contributor.authorAbang, Mathew M.
dc.contributor.authorAbraham, W.R.
dc.contributor.authorAsiedu, Robert
dc.contributor.authorHoffmann, P.
dc.contributor.authorWolf, G.
dc.contributor.authorWinter, S.
dc.date.accessioned2019-12-04T11:11:42Z
dc.date.available2019-12-04T11:11:42Z
dc.date.issued2009
dc.identifier.citationAbang, M.M., Abraham, W.R., Asiedu, R., Hoffmann, P., Wolf, G. & Winter, S. (2009). Secondary metabolite profile and phytotoxic activity of genetically distinct forms of Colletotrichum gloeosporioides from yam (Dioscorea spp.). Mycological Research, 113(1), 130-140.
dc.identifier.issn0953-7562
dc.identifier.urihttps://hdl.handle.net/20.500.12478/2481
dc.description.abstractHighly virulent, slow-growing grey (SGG); moderately virulent, fast-growing salmon (FGS); and avirulent/weakly virulent, fast-growing grey (FGG) forms of Colletotrichum gloeosporioides have been described from yam (Dioscorea spp.), but little is known about their chemodiversity or the role of toxins in their pathogenesis. Secondary metabolite profiles in high performance tlc (hptlc) showed that the pathogenic SGG and FGS forms have a chemotype (A or B) that is distinct from the non-pathogenic FGG form (chemotype C). Crude extracts of 35-d-old Czapek–Dox yeast broth cultures of FGS and SGG isolates caused tissue necrosis on treated yam leaves but not those of FGG isolates. Extracts from uninoculated broth cultures showed no phytotoxic activity. Toxicity of the culture filtrate was not host specific and toxic substances were thermostable. Dioscorea genotypes with varying levels of resistance to anthracnose differed in their sensitivity to crude toxin extract of FGS (Cg33) and SGG (Cg25) isolates, indicating that these extracts may be useful in evaluating host resistance to anthracnose in vitro. Analysis of two toxin fractions unique to the pathogenic FGS and SGG forms using hlpc, mass spectrometry and nuclear magnetic resonance suggested the presence of a low molecular weight amide peptide. However, possibly due to low yield and the presence of impurities, the chemical structure of the compound(s) could not be fully elucidated.
dc.description.sponsorshipGerman Academic Exchange Service
dc.format.extent130-140
dc.language.isoen
dc.subjectAnthracnose
dc.subjectChemotype
dc.subjectChromatography
dc.subjectColletotrichum Gloeosporioides
dc.subjectDioscorea Spp.
dc.subjectMass Spectrometry
dc.subjectMetabolite Profiling
dc.subjectNmr
dc.subjectPhytotoxin
dc.titleSecondary metabolite profile and phytotoxic activity of genetically distinct forms of Colletotrichum gloeosporioides from yam (Dioscorea spp.)
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.affiliationDeutsche Sammlung von Mikroorganismen und Zellkulturen
cg.contributor.affiliationHelmholtz Center for Infection Research
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationGeorg-August-Universität Göttingen
cg.coverage.regionAcp
cg.coverage.regionEurope
cg.coverage.countryGermany
cg.authorship.typesCGIAR and advanced research institute
cg.iitasubjectYam
cg.iitasubjectPlant Diseases
cg.iitasubjectTissue Culture
cg.iitasubjectGenetic Improvement
cg.journalMycological Research
cg.howpublishedFormally Published
cg.accessibilitystatusLimited Access
local.dspaceid93232
cg.identifier.doihttps://dx.doi.org/10.1016/j.mycres.2008.09.004


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