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    Estimation of the nuclear DNA content in some representative of genus Dioscorea

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    U09ArtObidiegwuEstimationNothomDev.pdf (141.8Kb)
    Date
    2009
    Author
    Obidiegwu, J.E.
    Rodríguez, E.
    Ene-Obong, E.E.
    Loureiro, J.
    Muoneke, C.O.
    Santos, C.
    Kolesnikova-Allen, M.
    Asiedu, Robert
    Type
    Journal Article
    Metadata
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    Abstract/Description
    Nuclear DNA content of 54 accessions of 6 Dioscorea species collected from West African countries were determined using flow cytometry. Observed ploidy levels are diploid (2x), triploid (3x), tetraploid (4x), hexaploid (6x) and octoploid (8x). DNA index varied from 0.36 to 1.54 depending on the cultivar and considered specie. Estimated nuclear DNA sizes ranged from 0.702 ± 0.004 pg for G1 nuclei of diploid Dioscorea dumetorum to 2.573 ± 0.020 pg for G1 nuclei of octoploid Dioscorea cayenensis. Our result showed that there is a decrease in genome size with higher ploidy level. The study contributes a step towards a genome understanding of this group of plants
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/2521
    IITA Subjects
    Yam; Genetic Improvement
    Agrovoc Terms
    Dioscorea; Dna Content; Flow Cytometry; Genome; Ploidy Level
    Regions
    Africa; West Africa
    Countries
    Togo; Sierra Leone; Benin; Cote D'Ivoire; Nigeria; Ghana
    Journals
    Scientific Research and Essays
    Collections
    • Journal and Journal Articles5283
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