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Estimation of the nuclear DNA content in some representative of genus Dioscorea

dc.contributor.authorObidiegwu, J.E.
dc.contributor.authorRodríguez, E.
dc.contributor.authorEne-Obong, E.E.
dc.contributor.authorLoureiro, J.
dc.contributor.authorMuoneke, C.O.
dc.contributor.authorSantos, C.
dc.contributor.authorKolesnikova-Allen, M.
dc.contributor.authorAsiedu, Robert
dc.date.accessioned2019-12-04T11:11:55Z
dc.date.available2019-12-04T11:11:55Z
dc.date.issued2009
dc.identifier.citationObidiegwu, J.E., Rodriguez, E., Ene-Obong, E.E., Loureiro, J., Muoneke, C.O., Santos, C., ... & Asiedu, R. (2009). Estimation of the nuclear DNA content in some representative of genus Dioscorea. Scientific Research and Essays, 4(5), 448-452.
dc.identifier.issn1992-2248
dc.identifier.urihttps://hdl.handle.net/20.500.12478/2521
dc.description.abstractNuclear DNA content of 54 accessions of 6 Dioscorea species collected from West African countries were determined using flow cytometry. Observed ploidy levels are diploid (2x), triploid (3x), tetraploid (4x), hexaploid (6x) and octoploid (8x). DNA index varied from 0.36 to 1.54 depending on the cultivar and considered specie. Estimated nuclear DNA sizes ranged from 0.702 ± 0.004 pg for G1 nuclei of diploid Dioscorea dumetorum to 2.573 ± 0.020 pg for G1 nuclei of octoploid Dioscorea cayenensis. Our result showed that there is a decrease in genome size with higher ploidy level. The study contributes a step towards a genome understanding of this group of plants
dc.description.sponsorshipKirkhouse Trust
dc.format.extent448-452
dc.language.isoen
dc.subjectDioscorea
dc.subjectDna Content
dc.subjectFlow Cytometry
dc.subjectGenome
dc.subjectPloidy Level
dc.titleEstimation of the nuclear DNA content in some representative of genus Dioscorea
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationNational Root Crops Research Institute, Nigeria
cg.contributor.affiliationMichael Okpara University of Agriculture
cg.contributor.affiliationUniversity of Aveiro
cg.contributor.affiliationTun Abdul Razak Research Centre
cg.coverage.regionAfrica
cg.coverage.regionWest Africa
cg.coverage.countryTogo
cg.coverage.countrySierra Leone
cg.coverage.countryBenin
cg.coverage.countryCote D'Ivoire
cg.coverage.countryNigeria
cg.coverage.countryGhana
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectYam
cg.iitasubjectGenetic Improvement
cg.journalScientific Research and Essays
cg.howpublishedFormally Published
cg.accessibilitystatusOpen Access
local.dspaceid93272


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