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    Application of Hosaka and DJ Kim Whatmann paper protocols for rapid isolation of cowpea DNA

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    U09ArtYusufApplicationInthomDev.pdf (184.8Kb)
    Date
    2009
    Author
    Yusuf, M.
    Muranaka, S.
    Zaria, A.A.
    Type
    Journal Article
    Target Audience
    Scientists
    Metadata
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    Abstract/Description
    The traditional liquid nitrogen DNA extraction method is expensive and tedious. There is, therefore, the need for cheaper and faster methods of DNA extraction for efficient application of marker assisted selection (MAS) for breeding for striga resistance in cowpea to be able to handle large number of samples at a time. Two DNA extraction methods; Hosaka (2004) and DJ Kim Whatmann paper (unpublished) were tested on cowpea line IT06K-5-83 under different conditions. The results revealed that both methods with modifications can work with cowpea. However, Hosaka (2004) method seems to be more promising than the other method for DNA extraction in cowpea because it gives better and more consistent DNA band.
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/2590
    IITA Subjects
    Cowpea; Genetic Improvement; Plant Production; Plant Genetic Resources; Plant Breeding; Research Method; Nutrition
    Agrovoc Terms
    Striga Resistance; Dna Extraction Methods; Marker Assisted Selection; Cowpea Dna; Rapid Isolation; Liquid Nitrogen; Ethanol And Vortexing
    Regions
    Africa; West Africa
    Countries
    Nigeria
    Journals
    African Journal of Biotechnology
    Collections
    • Journal and Journal Articles4835
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