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Application of Hosaka and DJ Kim Whatmann paper protocols for rapid isolation of cowpea DNA

dc.contributor.authorYusuf, M.
dc.contributor.authorMuranaka, S.
dc.contributor.authorZaria, A.A.
dc.date.accessioned2019-12-04T11:13:59Z
dc.date.available2019-12-04T11:13:59Z
dc.date.issued2009
dc.identifier.citationYusuf, M., Muranaka, S. & Zaria, A.A. (2009). Application of Hosaka and DJ Kim Whatmann paper protocols for rapid isolation of cowpea DNA. African Journal of Biotechnology, 8(23), 6518-6520.
dc.identifier.issn1684-5315
dc.identifier.urihttps://hdl.handle.net/20.500.12478/2590
dc.description.abstractThe traditional liquid nitrogen DNA extraction method is expensive and tedious. There is, therefore, the need for cheaper and faster methods of DNA extraction for efficient application of marker assisted selection (MAS) for breeding for striga resistance in cowpea to be able to handle large number of samples at a time. Two DNA extraction methods; Hosaka (2004) and DJ Kim Whatmann paper (unpublished) were tested on cowpea line IT06K-5-83 under different conditions. The results revealed that both methods with modifications can work with cowpea. However, Hosaka (2004) method seems to be more promising than the other method for DNA extraction in cowpea because it gives better and more consistent DNA band.
dc.format.extent6518-6520
dc.language.isoen
dc.subjectStriga Resistance
dc.subjectDna Extraction Methods
dc.subjectMarker Assisted Selection
dc.subjectCowpea Dna
dc.subjectRapid Isolation
dc.subjectLiquid Nitrogen
dc.subjectEthanol And Vortexing
dc.titleApplication of Hosaka and DJ Kim Whatmann paper protocols for rapid isolation of cowpea DNA
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.affiliationAhmadu Bello University
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.coverage.regionAfrica
cg.coverage.regionWest Africa
cg.coverage.countryNigeria
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectCowpea
cg.iitasubjectGenetic Improvement
cg.iitasubjectPlant Production
cg.iitasubjectPlant Genetic Resources
cg.iitasubjectPlant Breeding
cg.iitasubjectResearch Method
cg.iitasubjectNutrition
cg.journalAfrican Journal of Biotechnology
cg.howpublishedFormally Published
cg.accessibilitystatusOpen Access
local.dspaceid93394
cg.targetaudienceScientists


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