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    Diversity of begomoviruses associated with mosaic disease of cultivated cassava (Manihot esculenta Crantz) and its wild relative (Manihot glaziovii Mull. Arg.) in Uganda

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    Date
    2008
    Author
    Sserubombwe, W.S.
    Briddon, R.W.
    Baguma, Yona K.
    Ssemakula, G.N.
    Bull, S.E.
    Bua, A.
    Alicai, Titus
    Omongo, C.
    Otim-Nape, G.W.
    Stanley, J.
    Type
    Journal Article
    Metadata
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    Abstract/Description
    Cassava (Manihot esculenta) growing in Uganda during 2001–2002 has been screened for the presence of begomoviruses using PCR-RFLP, cloning full-length genomic components and nucleotide sequence analysis. In contrast with a recent survey in neighbouring Kenya, which identified three distinct strains of East African cassava mosaic virus (EACMV, EACMV-UG and EACMV-KE2) as well as East African cassava mosaic Zanzibar virus and the new species East African cassava mosaic Kenya virus, only EACMV-UG and, to a lesser extent, African cassava mosaic virus (ACMV) were found associated with cassava in Uganda. The integrity of the cloned genomic components of representative virus isolates was confirmed by demonstrating their infectivity in Nicotiana benthamiana and cassava using biolistic inoculation, providing a convenient means to screen cassava varieties for disease resistance. Both EACMV-UG and ACMV were also associated with Manihot glaziovii. Infectivity studies using cloned components confirmed that viruses from one host could infect the other, suggesting that this wild relative of cassava might be a reservoir host for the disease. The relatively low level of diversity of begomoviruses associated with cassava mosaic disease in Uganda is consistent with reports that EACMV-UG has displaced other begomovirus species and strains during the recent epidemic that swept through the country.
    https://dx.doi.org/10.1099/vir.0.83637-0
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/2813
    Digital Object Identifier (DOI)
    https://dx.doi.org/10.1099/vir.0.83637-0
    IITA Subjects
    Cassava; Plant Production; Food Security; Farm Management; Plant Diseases; Plant Health; Disease Control; Genetic Improvement; Pests Of Plants; Plant Breeding; Plant Genetic Resources
    Agrovoc Terms
    African Cassava Mosaic Virus; Begomovirus; Dna-A Segment; East African Cassava Mosaic Virus; African Cassava Mosaic Virus; Nucleotide
    Regions
    Acp; Africa; Europe; East Africa
    Countries
    United Kingdom; Uganda
    Collections
    • Journal and Journal Articles4836
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