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Diversity of begomoviruses associated with mosaic disease of cultivated cassava (Manihot esculenta Crantz) and its wild relative (Manihot glaziovii Mull. Arg.) in Uganda

dc.contributor.authorSserubombwe, W.S.
dc.contributor.authorBriddon, R.W.
dc.contributor.authorBaguma, Yona K.
dc.contributor.authorSsemakula, G.N.
dc.contributor.authorBull, S.E.
dc.contributor.authorBua, A.
dc.contributor.authorAlicai, Titus
dc.contributor.authorOmongo, C.
dc.contributor.authorOtim-Nape, G.W.
dc.contributor.authorStanley, J.
dc.date.accessioned2019-12-04T11:14:37Z
dc.date.available2019-12-04T11:14:37Z
dc.date.issued2008
dc.identifier.citationSserubombwe, W.S., Briddon, R.W., Baguma, Y.K., Ssemakula, G.N., Bull, S.E., Bua, A., ... & Stanley, J. (2008). Diversity of begomoviruses associated with mosaic disease of cultivated cassava (Manihot esculenta Crantz) and its wild relative (Manihot glaziovii Müll. Arg.) in Uganda. Journal of General Virology, 89(7), 1759-1769.
dc.identifier.issn0022-1317
dc.identifier.urihttps://hdl.handle.net/20.500.12478/2813
dc.description.abstractCassava (Manihot esculenta) growing in Uganda during 2001–2002 has been screened for the presence of begomoviruses using PCR-RFLP, cloning full-length genomic components and nucleotide sequence analysis. In contrast with a recent survey in neighbouring Kenya, which identified three distinct strains of East African cassava mosaic virus (EACMV, EACMV-UG and EACMV-KE2) as well as East African cassava mosaic Zanzibar virus and the new species East African cassava mosaic Kenya virus, only EACMV-UG and, to a lesser extent, African cassava mosaic virus (ACMV) were found associated with cassava in Uganda. The integrity of the cloned genomic components of representative virus isolates was confirmed by demonstrating their infectivity in Nicotiana benthamiana and cassava using biolistic inoculation, providing a convenient means to screen cassava varieties for disease resistance. Both EACMV-UG and ACMV were also associated with Manihot glaziovii. Infectivity studies using cloned components confirmed that viruses from one host could infect the other, suggesting that this wild relative of cassava might be a reservoir host for the disease. The relatively low level of diversity of begomoviruses associated with cassava mosaic disease in Uganda is consistent with reports that EACMV-UG has displaced other begomovirus species and strains during the recent epidemic that swept through the country.
dc.description.sponsorshipEuropean Union
dc.language.isoen
dc.subjectAfrican Cassava Mosaic Virus
dc.subjectBegomovirus
dc.subjectDna-A Segment
dc.subjectEast African Cassava Mosaic Virus
dc.subjectAfrican Cassava Mosaic Virus
dc.subjectNucleotide
dc.titleDiversity of begomoviruses associated with mosaic disease of cultivated cassava (Manihot esculenta Crantz) and its wild relative (Manihot glaziovii Mull. Arg.) in Uganda
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.affiliationNorwich Research Park
cg.contributor.affiliationNational Crops Resources Research Institute, Uganda
cg.coverage.regionAcp
cg.coverage.regionAfrica
cg.coverage.regionEurope
cg.coverage.regionEast Africa
cg.coverage.countryUnited Kingdom
cg.coverage.countryUganda
cg.isijournalISI Journal
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectCassava
cg.iitasubjectPlant Production
cg.iitasubjectFood Security
cg.iitasubjectFarm Management
cg.iitasubjectPlant Diseases
cg.iitasubjectPlant Health
cg.iitasubjectDisease Control
cg.iitasubjectGenetic Improvement
cg.iitasubjectPests Of Plants
cg.iitasubjectPlant Breeding
cg.iitasubjectPlant Genetic Resources
cg.accessibilitystatusLimited Access
local.dspaceid93914
cg.identifier.doihttps://dx.doi.org/10.1099/vir.0.83637-0


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