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    Multiplex PCR for the detection of African cassava mosaic virus and East African cassava mosaic Cameroon virus in cassava

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    alabi-multiplex-2008.pdf (1.149Mb)
    Date
    2008
    Author
    Alabi, O.J.
    Kumar, P.L.
    Naidu, R.A.
    Type
    Journal Article
    Metadata
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    Abstract/Description
    A multiplex PCR was developed for simultaneous detection of African cassava mosaic virus (ACMV) and East African cassava mosaic Cameroon virus (EACMCV) in cassava affected with cassava mosaic disease (CMD). One set of three primers consisting of an upstream primer common for both viruses and two down stream virus-specific primers were designed for simultaneous amplification of 368 base pair (bp) and 650 bp DNA fragments specific to the replicase gene of ACMV and EACMCV, respectively. Similarly, a second set of three primerswere designed for simultaneous amplification of 540 bp and 655 bp fragments specific to the coat protein gene of EACMCV and ACMV, respectively. Primers that can amplify a 171 bp fragment of the large subunit of ribulose bisphosphate carboxylase oxygenase Lwere included as an internal control in these assays to determine the reliability of multiplex PCR. A simplified, cost-effective and rapid sample preparation method was adapted in place of the conventional plant DNA extraction procedure for multiplex PCR detection of ACMV and EACMCV. The method was validated using CMD-infected cassava samples obtained from farmers’ fields in Nigeria. The multiplex PCR is useful for reliable assessment of the prevalence of CMBs in epidemiological studies and for crop improvement and phytosanitary programs in African countries.
    https://dx.doi.org/10.1016/j.jviromet.2008.08.008
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/2894
    Digital Object Identifier (DOI)
    https://dx.doi.org/10.1016/j.jviromet.2008.08.008
    IITA Subjects
    Disease Control; Cassava; Genetic Improvement; Plant Production; Pests Of Plants; Plant Diseases; Plant Health; Plant Breeding; Plant Genetic Resources; Farm Management; Food Security
    Agrovoc Terms
    Multiplex Pcr; Begomovirus; African Cassava Mosaic Virus; Sub Saharan Africa; Whitefly; African Cassava Mosaic Virus; East African Cassava Mosaic Cameroon Virus; Begomovirus
    Regions
    Acp; Africa; North America; West Africa
    Countries
    United States; Nigeria
    Collections
    • Journal and Journal Articles4835

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      Alternate hosts of African cassava mosaic virus and East African cassava mosaic Cameroon virus in Nigeria 

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      Cassava mosaic disease (CMD) caused by African cassava mosaic virus (ACMV) and East African cassava mosaic Cameroon virus (EACMCV) is the major constraint to cassava production in Nigeria. Sequences of the DNA-A component of ACMV and EACMCV isolates from leguminous plant species (Senna occidentalis, Leucana leucocephala and Glycine max), castor oil plant (Ricinus communis), a weed host (Combretum confertum) and a wild species of cassava (Manihot glaziovii) were determined. All ACMV isolates from ...
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      The occurrence of African cassava mosaic virus and East African cassava mosaic Cameroon virus in natural hosts other than cassava in Nigeria 

      Alabi, O.J.; Ogbe, F.O.; Bandyopadhyay, Ranajit; Dixon, Alfred G.O.; Hughes, J.; Naidu, R.A. (2007)
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