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Multiplex PCR for the detection of African cassava mosaic virus and East African cassava mosaic Cameroon virus in cassava

dc.contributor.authorAlabi, O.J.
dc.contributor.authorKumar, P.L.
dc.contributor.authorNaidu, R.A.
dc.date.accessioned2019-12-04T11:14:59Z
dc.date.available2019-12-04T11:14:59Z
dc.date.issued2008
dc.identifier.citationAlabi, O.J., Kumar, P.L. & Naidu, R.A. (2008). Multiplex PCR for the detection of African cassava mosaic virus and East African cassava mosaic Cameroon virus in cassava. Journal of Virological Methods, 154(1), 111-120.
dc.identifier.issn0166-0934
dc.identifier.urihttps://hdl.handle.net/20.500.12478/2894
dc.description.abstractA multiplex PCR was developed for simultaneous detection of African cassava mosaic virus (ACMV) and East African cassava mosaic Cameroon virus (EACMCV) in cassava affected with cassava mosaic disease (CMD). One set of three primers consisting of an upstream primer common for both viruses and two down stream virus-specific primers were designed for simultaneous amplification of 368 base pair (bp) and 650 bp DNA fragments specific to the replicase gene of ACMV and EACMCV, respectively. Similarly, a second set of three primerswere designed for simultaneous amplification of 540 bp and 655 bp fragments specific to the coat protein gene of EACMCV and ACMV, respectively. Primers that can amplify a 171 bp fragment of the large subunit of ribulose bisphosphate carboxylase oxygenase Lwere included as an internal control in these assays to determine the reliability of multiplex PCR. A simplified, cost-effective and rapid sample preparation method was adapted in place of the conventional plant DNA extraction procedure for multiplex PCR detection of ACMV and EACMCV. The method was validated using CMD-infected cassava samples obtained from farmers’ fields in Nigeria. The multiplex PCR is useful for reliable assessment of the prevalence of CMBs in epidemiological studies and for crop improvement and phytosanitary programs in African countries.
dc.description.sponsorshipUnited States Agency for International Development
dc.language.isoen
dc.subjectMultiplex Pcr
dc.subjectBegomovirus
dc.subjectAfrican Cassava Mosaic Virus
dc.subjectSub Saharan Africa
dc.subjectWhitefly
dc.subjectAfrican Cassava Mosaic Virus
dc.subjectEast African Cassava Mosaic Cameroon Virus
dc.subjectBegomovirus
dc.titleMultiplex PCR for the detection of African cassava mosaic virus and East African cassava mosaic Cameroon virus in cassava
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.affiliationWashington State University
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.coverage.regionAcp
cg.coverage.regionAfrica
cg.coverage.regionNorth America
cg.coverage.regionWest Africa
cg.coverage.countryUnited States
cg.coverage.countryNigeria
cg.isijournalISI Journal
cg.authorship.typesCGIAR and advanced research institute
cg.iitasubjectDisease Control
cg.iitasubjectCassava
cg.iitasubjectGenetic Improvement
cg.iitasubjectPlant Production
cg.iitasubjectPests Of Plants
cg.iitasubjectPlant Diseases
cg.iitasubjectPlant Health
cg.iitasubjectPlant Breeding
cg.iitasubjectPlant Genetic Resources
cg.iitasubjectFarm Management
cg.iitasubjectFood Security
cg.accessibilitystatusLimited Access
local.dspaceid93995
cg.identifier.doihttps://dx.doi.org/10.1016/j.jviromet.2008.08.008


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