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    Rapid and efficient production of transgenic East African highland banana (Musa spp.) using intercalary meristematic tissues

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    tripathi-rapid-2008.pdf (637.9Kb)
    Date
    2008-05
    Author
    Tripathi, L.
    Tripathi, J.N.
    Tushemereirwe, W.K.
    Type
    Journal Article
    Metadata
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    Abstract/Description
    East Africa is the largest banana producing and consuming region in Africa. In particular, the East African Highland Banana serves as the major staple crop of countries like Uganda, but production is constrained by a number of serious pests and diseases. Banana breeding is a very difficult and slow process, so genetic engineering offers an alternative approach to improvement. A transformation system using intercalary meristematic tissues was developed using Agrobacterium strain EHA105 harboring the binary vector pCAMBIA2301 containing the gusA reporter gene and nptII as selectable marker. In this paper, a new transformation protocol is described that yields kanamycin-resistant, GUSexpressing banana plants from roughly 10% of the initial explants. The resulting fully-rooted transgenic plants do not appear to be chimeras since they can be stably propagated, GUS activity is observed uniformly throughout the plants including the germline cells of the meristem, and PCR and Southern blots indicate stable integration of the genes into the genome.
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/2923
    IITA Subjects
    Smallholder Farmers; Banana; Pests Of Plants; Plant Production; Food Security; Plant Genetic Resources; Handling, Transport, Storage And Protection Of Agricultural Products; Livelihoods; Disease Control; Nutrition; Genetic Improvement; Plant Diseases
    Agrovoc Terms
    Agrobacterium; Intercalary Meristem; Genetic Transformation; Bananas; East African Highland Bananas; Xanthomonas; Banana Streak Virus
    Regions
    Africa; East Africa
    Countries
    Uganda
    Collections
    • Journal and Journal Articles4842
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