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Rapid and efficient production of transgenic East African highland banana (Musa spp.) using intercalary meristematic tissues

dc.contributor.authorTripathi, L.
dc.contributor.authorTripathi, J.N.
dc.contributor.authorTushemereirwe, W.K.
dc.date.accessioned2019-12-04T11:15:08Z
dc.date.available2019-12-04T11:15:08Z
dc.date.issued2008-05
dc.identifier.citationTripathi, L., Tripathi, J.N. & Tushemereirwe, W.K. (2008). Rapid and efficient production of transgenic East African Highland Banana (Musa spp.) using intercalary meristematic tissues. African Journal of Biotechnology, 7(10), 1438-1445.
dc.identifier.issn1684-5315
dc.identifier.urihttps://hdl.handle.net/20.500.12478/2923
dc.description.abstractEast Africa is the largest banana producing and consuming region in Africa. In particular, the East African Highland Banana serves as the major staple crop of countries like Uganda, but production is constrained by a number of serious pests and diseases. Banana breeding is a very difficult and slow process, so genetic engineering offers an alternative approach to improvement. A transformation system using intercalary meristematic tissues was developed using Agrobacterium strain EHA105 harboring the binary vector pCAMBIA2301 containing the gusA reporter gene and nptII as selectable marker. In this paper, a new transformation protocol is described that yields kanamycin-resistant, GUSexpressing banana plants from roughly 10% of the initial explants. The resulting fully-rooted transgenic plants do not appear to be chimeras since they can be stably propagated, GUS activity is observed uniformly throughout the plants including the germline cells of the meristem, and PCR and Southern blots indicate stable integration of the genes into the genome.
dc.language.isoen
dc.subjectAgrobacterium
dc.subjectIntercalary Meristem
dc.subjectGenetic Transformation
dc.subjectBananas
dc.subjectEast African Highland Bananas
dc.subjectXanthomonas
dc.subjectBanana Streak Virus
dc.titleRapid and efficient production of transgenic East African highland banana (Musa spp.) using intercalary meristematic tissues
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationKawanda Agricultural Research Institute, Uganda
cg.coverage.regionAfrica
cg.coverage.regionEast Africa
cg.coverage.countryUganda
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectSmallholder Farmers
cg.iitasubjectBanana
cg.iitasubjectPests Of Plants
cg.iitasubjectPlant Production
cg.iitasubjectFood Security
cg.iitasubjectPlant Genetic Resources
cg.iitasubjectHandling, Transport, Storage And Protection Of Agricultural Products
cg.iitasubjectLivelihoods
cg.iitasubjectDisease Control
cg.iitasubjectNutrition
cg.iitasubjectGenetic Improvement
cg.iitasubjectPlant Diseases
cg.accessibilitystatusOpen Access
local.dspaceid94024


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